Fragile-X-Digoxigenin

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货号：S1120

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Catalogue Number	S1120
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Fragile X CHEMI™ DNA Probe (digoxigenin-labeled)
Overview	The Fragile X Syndrome is identified by an ampiflication of the FMR1 (CGG)n repeat region. The same results are obtained as when using the standard P32-labeled probes, but without radioactivity. The amplification of the FMR-1 gene is detected on Southern blots by an increase in the band sizes. Also provided is a ready-to-use mixture of unlabeled and digoxigenin-labeled Lambda DNA/Hind III Molecular Weight Markers for convenient visualization on both ethidium bromide-stained agarose gels and Southern blots.

References

Product Information
Detection method	Chemiluminescence

Applications
Application Notes	Fragile X CHEMI™ is intended for Research Use Only. For best results, target human genomic DNA should be double digested, to completion, with the restriction enzymes EcoR I and either Sac II, Nru I, or Eag I. S1120-1 Fragile X (pFxa1NHE) DNA Probe Locus: FMR1 Volume: 250 μl Concentration : 10ng/μl; 25 μl per 10 ml of hybridization solution. Sufficient probe to make 100 ml of hybridization solution at a probe concentration of 25 ng/ml. (equal to processing 1500 cm2) Restriction Map: see insert. S1120-2 Lambda/Hind III Molecular Weight Markers Fragile X CHEMI™ Molecular Weight Markers are Lambda DNA cut with Hind III. Each vial contains a ready-to-use mixture of unlabeled and digoxigenin-labeled DNA fragments in gel loading solution. The unlabeled markers are included for convenient visualization on agarose gel after staining with ethidium bromide. The fragment sizes are: 23.1, 9.4, 6.6, 4.4, 2.3, 2.0, 1.0, 0.56, 0.125 kb. The 0.125 kb fragment may be difficult to detect, and will likely electrophorese off the end of the gel. Volume: 800 μl. Sufficient for 20 lanes, 10 gels with 2 lanes per gel, 40 μl per lane. Materials Required but not Provided 20X PBS Tween®-20 1M MgCl2 X-ray film/cassette(s) 20X SSC Packets 20% SDS Report Cover acetate sheets or plastic wrap Instructions for Use Molecular Weight Markers 1. Thaw contents of S1120-2 at room temperature or 37°C and aliquot 40 μl per lane into a separate microcentrifuge tube. 2. Incubate the microcentrifuge tube containing the aliquoted markers at 65°C for 3-5 minutes. Load 40 μl per lane onto an agarose gel and proceed with electrophoresis and transfer. Do not incubate the stock tube of markers at 65°C. Probe Hybridization Procedural Cautions Temperature and buffer salt concentration (stringency) of hybridization and washing are important, as lower stringency can result in non-specific binding of the probe to other sequences, and higher stringency can result in lack of signal. Additional Comments 1. The Fragile X CHEMI™ DNA Probe is optimized for chemiluminescent detection. 2. Use 10 μg of restriction enzyme digested human DNA per lane.

Applications

Application Notes

Fragile X CHEMI™ is intended for Research Use Only. For best results, target human genomic DNA should be double digested, to completion, with the restriction enzymes EcoR I and either Sac II, Nru I, or Eag I.

S1120-1 Fragile X (pFxa1NHE) DNA Probe

Locus: FMR1

Volume: 250 μl Concentration : 10ng/μl; 25 μl per 10 ml of hybridization solution. Sufficient probe to make 100 ml of hybridization solution at a probe concentration of 25 ng/ml. (equal to processing 1500 cm2)

Restriction Map: see insert.

S1120-2 Lambda/Hind III Molecular Weight Markers

Fragile X CHEMI™ Molecular Weight Markers are Lambda DNA cut with Hind III. Each vial contains a ready-to-use mixture of unlabeled and digoxigenin-labeled DNA fragments in gel loading solution. The unlabeled markers are included for convenient visualization on agarose gel after staining with ethidium bromide. The fragment sizes are: 23.1, 9.4, 6.6, 4.4, 2.3, 2.0, 1.0, 0.56, 0.125 kb. The 0.125 kb fragment may be difficult to detect, and will likely electrophorese off the end of the gel.

Volume: 800 μl. Sufficient for 20 lanes, 10 gels with 2 lanes per gel, 40 μl per lane.

Materials Required but not Provided

20X PBS Tween®-20

1M MgCl2 X-ray film/cassette(s)

20X SSC Packets

20% SDS

Report Cover acetate sheets or plastic wrap

Instructions for Use

Molecular Weight Markers

1. Thaw contents of S1120-2 at room temperature or 37°C and aliquot 40 μl per lane into a separate microcentrifuge tube.

2. Incubate the microcentrifuge tube containing the aliquoted markers at 65°C for 3-5 minutes. Load 40 μl per lane onto an agarose gel and proceed with electrophoresis and transfer.

Do not incubate the stock tube of markers at 65°C.

Probe Hybridization

Procedural Cautions

Temperature and buffer salt concentration (stringency) of hybridization and washing are important, as lower stringency can result in non-specific binding of the probe to other sequences, and higher stringency can result in lack of signal.

Additional Comments

1. The Fragile X CHEMI™ DNA Probe is optimized for chemiluminescent detection.

2. Use 10 μg of restriction enzyme digested human DNA per lane.

Biological Information
Species Reactivity	Human

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Cell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended Storage The Fragile X CHEMI™ DNA Probe and Molecular Weight Markers should be stored at -15°C to -25°C.

Packaging Information
Material Size	10 assays

Transport Information

Supplemental Information

Specifications

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