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Chk X Aquaporin-7

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货号：AB3076

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R ELISA, ICC, IHC, WB Ch Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB3076
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Aquaporin 7 Antibody
Alternate Names
  • AQP7
References
Product Information
Format Affinity Purified
Presentation Liquid in 100 mM Tris, pH 7.5 containing 0.2% BSA as stabilizer and 0.05% sodium azide.
Applications
Application This Anti-Aquaporin 7 Antibody is validated for use in ELISA, IC, IH, WB for the detection of Aquaporin 7.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1-10 μg/mL using Chemiluminescence technique


Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.


Immunocytochemistry: 2-10 μg/mL. Adherent cells can be fixed in 50% methanol-50% acetone or 1% paraformaldehyde.


ELISA: 1:100,000 using 50-100 ng of control peptide per well.


Optimal working dilutions must be determined by end user.
Biological Information
Immunogen An 18 AA synthetic peptide within the N-terminal domain of rat Aquaporin-7 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Chicken
Specificity Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-7 (rAQP7, 269aa) is abundantly expressed in testis. It is involved in water, glycerol and urea transport. Aquaporin-7 is also found in adipose tissue, kidney, and heart. Aquaporin-7 has been localized to in the spermatids and at maturing sperms. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary Aquaporins/major intrinsic protein (MIP) are a family of water-selective membrane channels. Aquaporin 7 has greater sequence similarity with AQP3 and AQP9 and they may be a subfamily. Aquaporin 7 and AQP3 are at the same chromosomal location suggesting that 9p13 may be a site of an aquaporin cluster. Aquaporin 7 facilitates water, glycerol and urea transport. It may play an important role in sperm function.
Gene Symbol
  • AQP7
  • Aquaporin-7-like
  • AQP-7
  • AQPap
  • AQP9
  • MGC149555
  • Aquaporin-7
  • MGC149556
  • AQP7L
UniProt Number
UniProt Summary FUNCTION: SwissProt: O14520 # Forms a channel for water and glycerol.
SIZE: 342 amino acids; 37232 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: O14520 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro/Ala-Ala/Ser (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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