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Rat ACE

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号:AG782

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Key Applications Species Uni Prot Number
PC Rat P47820
Description
Catalogue Number AG782
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Angiotensin Converting Enzyme, rat
Overview ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase. ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vaso-depressor. ACE appears to play a key role in regulating vascular tone and remodeling the development of atherosclerotic lesions. Increasing evidence suggests that ACE plays an important role in vascular pathology. Recent studies shows two new functions of this important enzyme: 1) It enhances the presentation of endogenous antigens (in particularly HIV 1 gp160-derived peptide p18) to MHC class I-restricted T-lynphocytes (ref. 1); 2) Its amino-terminal domain cleaves effectively hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (ref. 2,3) which is involved in the control of hemapoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (ref. 4).
Alternate Names
  • ACE
  • CD143
  • DCP1
  • Peptidyl-dipeptidase A
  • Peptidase P
References
Product Information
Presentation Liquid in 100 mM phosphate buffered saline, 150 mM NaCl, pH 7.4 with 0.2mM CHAPS. Purity is >95% by SDS-PAGE when 10 μg was loaded. Contains no preservative.
Applications
Key Applications
  • Positive Control
Application Notes As a reference in SDS-PAGE and Western blotting [together with anti-ACE monoclonal (MAB4051) also available from Chemicon]. For Western blotting 500 ng/lane if gel is developed with anti-ACE MAB4051.

As a reference antigen for immunoprecipitation of ACE from various tissue samples (in combination with anti-ACE MAB4051).

As a source of pure ACE for different enzymatic studies of ACE (testing of ACE inhibitors, substrate specificity, kinetic measurements, etc.).

Optimal working concentration must be determined by the end user.
Biological Information
Concentration Refer to Certificate of Analysis for each individual lot of this enzyme.
Source Isolated from rat lung using affinity chromatography (ref. 5).
Specific Activity One unit will produce 1 μmoL of hippuric acid or His-Leu from Z-Phe-His-Leu per minute in 0.1 M phosphate buffer and 300 mM NaCl at pH 8.3 at 37°C.
Gene Symbol
  • ACE
  • DCP1
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 1 unit
Transport Information
Supplemental Information
Specifications

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