Anti-AMPK a 1

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Polyclonal Antibody

Description
Catalogue Number	07-350
Replaces	04-323
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-AMPK α1 Antibody
Alternate Names	5'-AMP-activated protein kinase, catalytic alpha-1 chain AMP -activate kinase alpha 1 subunit AMP-activated protein kinase, catalytic, alpha-1 AMPK alpha 1 AMPK alpha-1 chain, protein kinase AMP-activated, alpha 1 catalytic subunit
Background Information	5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt: Q13131; also known as EC:2.7.11.1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1) is encoded by the PRKAA1 (also known as AMPK1) gene (Gene ID: 5562) in human.AMPK is a heterotrimer composed of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). It also contains an autoinhibitory sequence that shows some sequence similarity with the ubiquitin-associated domains and it represses kinase activity. AMPK is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes, including inhibition of protein, carbohydrate and lipid biosynthesis, and reduces cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK is shown to regulate insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 and it stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter GLUT4 to the plasma membrane. In the liver it acts as a key regulator of glucose homeostasis by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. AMPK is activated by phosphorylation on Thr183. Binding of AMP to non-catalytic gamma subunit results in allosteric activation, inducing phosphorylation on Thr183. AMP-binding to gamma subunit also sustains activity by preventing dephosphorylation of Thr183.

Description

Catalogue Number

07-350

Replaces

04-323

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-AMPK α1 Antibody

Alternate Names

5'-AMP-activated protein kinase, catalytic alpha-1 chain
AMP -activate kinase alpha 1 subunit
AMP-activated protein kinase, catalytic, alpha-1
AMPK alpha 1
AMPK alpha-1 chain, protein kinase
AMP-activated, alpha 1 catalytic subunit

Background Information

5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt: Q13131; also known as EC:2.7.11.1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1) is encoded by the PRKAA1 (also known as AMPK1) gene (Gene ID: 5562) in human.AMPK is a heterotrimer composed of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). It also contains an autoinhibitory sequence that shows some sequence similarity with the ubiquitin-associated domains and it represses kinase activity. AMPK is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes, including inhibition of protein, carbohydrate and lipid biosynthesis, and reduces cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK is shown to regulate insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 and it stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter GLUT4 to the plasma membrane. In the liver it acts as a key regulator of glucose homeostasis by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. AMPK is activated by phosphorylation on Thr183. Binding of AMP to non-catalytic gamma subunit results in allosteric activation, inducing phosphorylation on Thr183. AMP-binding to gamma subunit also sustains activity by preventing dephosphorylation of Thr183.

Product Information
Format	Purified
Control	A431cell lysate, or lysate of HeLa cells treated with heat shock Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation	Protein A purified rabbit in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Product Information

Format

Purified

Control

A431cell lysate, or lysate of HeLa cells treated with heat shock
Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Presentation

Protein A purified rabbit in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Applications
Application	Anti-AMPK alpha 1, Cat. No. 07-350 is rabbit polyclonal antibody that detects 5'-AMP-activated protein kinase catalytic subunit alpha-1 and is tested for use in Western Blotting.
Key Applications	Western Blotting

Applications

Application

Anti-AMPK alpha 1, Cat. No. 07-350 is rabbit polyclonal antibody that detects 5'-AMP-activated protein kinase catalytic subunit alpha-1 and is tested for use in Western Blotting.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human AMPK catalytic subunit alpha-1.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This polyclonal antibody detects AMPK1 alpha subunit in human. It targets an epitope within 17 amino acids from the C-terminal half of human AMPK1.
Isotype	IgG
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006242
Entrez Gene Summary	The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol	PRKAA1 MGC57364 AMPK MGC33776 AMPKa1 AMPK1
Purification Method	Protein A chromatography
UniProt Number	Q13131
Molecular Weight	~63 kDa observed; 64.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human AMPK catalytic subunit alpha-1.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This polyclonal antibody detects AMPK1 alpha subunit in human. It targets an epitope within 17 amino acids from the C-terminal half of human AMPK1.

Isotype

IgG

Species Reactivity

Human

Species Reactivity Note

Human. Predicted to react with Mouse and Rat based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006242

Entrez Gene Summary

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene Symbol

PRKAA1
MGC57364
AMPK
MGC33776
AMPKa1
AMPK1

Purification Method

Protein A chromatography

UniProt Number

Q13131

Molecular Weight

~63 kDa observed; 64.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Product Usage Statements
Quality Assurance	Evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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