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Anti-MAP4 (rabbit polyclonal)

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货号：AB6020

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Eq, Ca, B WB, ICC Rb Purified Polyclonal Antibody
Description
Catalogue Number AB6020
Description Anti-MAP4 Antibody
Alternate Names
  • microtubule-associated protein 4
  • cdc kinase
  • MAP-4
Background Information Microtubule-associated protein 4 (MAP4) is a ubiquitous non-neuronal microtubule-associated protein. This protein binds, polymerizes, and stabilizes microtubules and is thought to regulate microtubule dynamics during the cell cycle. p34cdc2 kinase phosphorylation of MAP4 appears to regulate the control of microtubule reorganization at mitosis (Ookata, 1997).
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application This Anti-MAP4 Antibody is validated for use in WB, IC for the detection of MAP4.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Western blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected MAP4 in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MAP4 in HeLa, NIH/3T3, and A431 cells.
Biological Information
Immunogen KLH-conjugated peptide corresponding to the C-terminus of human MAP4.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Antibody recognizes the C-terminus of MAP4.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Horse
  • Canine
  • Bovine
Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with equine, canine, and bovine based upon 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol
  • MAP4
  • MAP-4
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

SUBUNIT STRUCTURE: Interacts with SEPT2; this interaction impedes tubulin-binding.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

SEQUENCE SIMILARITIES: Contains 4 Tau/MAP repeats.
Molecular Weight ~ 220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western blot in HeLa cell lysate.

Western blot Analysis: 0.05 µg/mL of this antibody detected MAP4 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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