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Qiagen 凯杰 Biosharp Omega

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ANTI-ER B, CT 200UG

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货号：07-359

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Analytes Available Detection Methods
KA, WB B-Raf Chemiluminescence
Description
Catalogue Number 17-359
Brand Family Upstate
Trade Name
  • Upstate
Description B-Raf Kinase Assay Kit, Chemiluminescence Detection
Overview Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user. For assay of B-Raf activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-B-Raf antibody (07-453).
References
Product Information
Components
  • B-Raf (δ1-415), active (Cat.# 14-530)
  • MEK1, unactive (Cat.# 14-420)
  • Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
  • Magnesium/ATP Cocktail (Cat.# 20-113)
  • Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Cat.# 07-461)
Detection method Chemiluminescence
Applications
Application Chemiluminscent detection kit intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user.
Key Applications
  • Kinase Assay
  • Western Blotting
Biological Information
Analytes Available
  • B-Raf
Entrez Gene Number
Gene Symbol
  • BRAF
  • B-raf-1
  • BRAF1
  • MGC126806
  • B-Raf
  • p94
  • MGC138284
  • RAFB1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance phosphorylation of MEK1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 kit
Material Package Kit capacity: 50 assays
Transport Information
Supplemental Information
Specifications

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