Anti-Histone H2B

Species Reactivity	Key Applications	Host	Format	Antibody Type
Ch, H	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Ch, H

Purified

Polyclonal Antibody

Description
Catalogue Number	07-371
Replaces	MABE15
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Histone H2B Antibody
Alternate Names	H2B Histone H2B H2B histone family, member Q histone 2 H2be histone cluster 2 H2be
Background Information	Histone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.

Description

Catalogue Number

07-371

Replaces

MABE15

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Histone H2B Antibody

Alternate Names

H2B
Histone H2B
H2B histone family, member Q
histone 2
H2be
histone cluster 2
H2be

Background Information

Histone H2B type 3-B (UniProt: Q8N257; also known as H2B type 12) is encoded by the HIST3H2BB gene (Gene ID: 128312) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Histone H2B is phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase and may be correlated with chromosome condensation. Histone H2B can undergo acetylation by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.

Product Information
Format	Purified
Control	Acid extract from either sodium butyrate or colcemid treated HeLa cells.
Presentation	Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.

Product Information

Format

Purified

Control

Acid extract from either sodium butyrate or colcemid treated HeLa cells.

Presentation

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, with 0.1% sodium azide and 30% glycerol.

Applications
Application	Anti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.
Key Applications	Western Blotting
Application Notes	Western Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.

Applications

Application

Anti-Histone H2B Antibody, Cat. No. 07-371, is a highly specific rabbit polyclonal antibody that targets Histone H2B and has been tested in Western Blotting.

Key Applications

Western Blotting

Application Notes

Western Blotting Analysis: 1-2 µg/mL from a representative lot detected Histone H2B in acid extract of HeLa cells treated with either sodium butyrate or colcemid.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.
Isotype	IgG
Species Reactivity	Chicken Human
Species Reactivity Note	Human, Chicken. Predicted to react with Yeast, Xenopus based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_778225
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Gene Symbol	GL105 H2B H2B-GL105 H2B.1 H2B.q H2B/q H2BFQ MGC119802 MGC119804 MGC129733 MGC129734 OTTHUMP00000013920
Purification Method	Protein A chromatography
UniProt Number	Q8N257
UniProt Summary	FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 126 amino acids; 13908 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination. SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
Molecular Weight	~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminus of human Histone H2B type 3-B.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This rabbit polyclonal antibody detects Histone H2B in human and chicken. It targets an epitope within 9 amino acids from the C-terminus region.

Isotype

IgG

Species Reactivity

Chicken
Human

Species Reactivity Note

Human, Chicken. Predicted to react with Yeast, Xenopus based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_778225

Entrez Gene Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.

Gene Symbol

GL105
H2B
H2B-GL105
H2B.1
H2B.q
H2B/q
H2BFQ
MGC119802
MGC119804
MGC129733
MGC129734
OTTHUMP00000013920

Purification Method

Protein A chromatography

UniProt Number

Q8N257

UniProt Summary

FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 126 amino acids; 13908 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.

Molecular Weight

~17 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Chicken Core Histone. Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in Chicken Core Histone.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H2B in Chicken Core Histone.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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