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Anti-Histamine Receptor H3 (rabbit polyclonal)

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货号：AB15860

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R, H FC, IH(P), WB Rb Serum Polyclonal Antibody
Description
Catalogue Number AB1560
Replaces MAB5330
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Opioid Receptor Antibody, δ, pain, NT
References
Product Information
Format Serum
Control
  • Spinal cord
Presentation UnPurified whole rabbit antiSerum containing no preservatives.
Applications
Application Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.

Reactive on paraffin embedded tissue.

Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.

FACS on human T-cells (Sharp, 2001)

Optimal working dilutions must be determined by the end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (without Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Biological Information
Immunogen Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
Epitope N-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
Species Reactivity
  • Mouse
  • Rat
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • OPRD1
  • OPRD
  • DOR-1
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P41143 # Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
SIZE: 372 amino acids; 40413 Da
SUBUNIT: Interacts with GPRASP1.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P41143 ## Belongs to the G-protein coupled receptor 1 family.
Molecular Weight 36 and 72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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