GRB2, agarose conjugate

Key Applications	Format
WB	Purified

Key Applications

Format

Purified

Description
Catalogue Number	14-1128
Description	Di-Ubiquitin (Linear) Recombinant Protein \| 14-1128
Background Information	The function, processing and ultimate fate of polyubiquitinylated proteins is implied to be determined by the linkage between adjacent ubiquitin molecules in the polyubiquitin chain. The post-translational modification of proteins with polyubiquitin chains occurs in a wide range of signalling pathways and is tightly regulated in order to ensure cellular homeostasis. Several ubiquitin polygenes are encoded in eukaryotic cells and undergo post-translational processing to generate the cellular source of free ubiquitin monomers. However, linear polyubiquitin chains have been shown to be assembled in vitro by an E3 ligase complex, known as the linear ubiquitin chain assembly complex (LUBAC), and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified in a number of proteins. Recent studies suggest that linear polyubiquitin modification of proteins may play an important role in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation. Aditionally, studies suggest a possible role for linear polyubiquitin modification in NF-κB pathway activation, involving linear polyubiquitinylation of NEMO.

Description

Catalogue Number

14-1128

Description

Di-Ubiquitin (Linear) Recombinant Protein | 14-1128

Background Information

The function, processing and ultimate fate of polyubiquitinylated proteins is implied to be determined by the linkage between adjacent ubiquitin molecules in the polyubiquitin chain. The post-translational modification of proteins with polyubiquitin chains occurs in a wide range of signalling pathways and is tightly regulated in order to ensure cellular homeostasis. Several ubiquitin polygenes are encoded in eukaryotic cells and undergo post-translational processing to generate the cellular source of free ubiquitin monomers. However, linear polyubiquitin chains have been shown to be assembled in vitro by an E3 ligase complex, known as the linear ubiquitin chain assembly complex (LUBAC), and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified in a number of proteins. Recent studies suggest that linear polyubiquitin modification of proteins may play an important role in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation. Aditionally, studies suggest a possible role for linear polyubiquitin modification in NF-κB pathway activation, involving linear polyubiquitinylation of NEMO.

Product Information
Format	Purified
Presentation	Liquid. 50 µg of Di-Ubiquitin reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM. General used concentrations vary from 10 – 50 μM. Buffer exchange using 3 kDa spin filters (or dialysis membrane) can be used to remove the DMSO if desired.

Product Information

Format

Purified

Presentation

Liquid. 50 µg of Di-Ubiquitin reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM. General used concentrations vary from 10 – 50 μM. Buffer exchange using 3 kDa spin filters (or dialysis membrane) can be used to remove the DMSO if desired.

Applications
Key Applications	Western Blotting
Application Notes	Liquid Chromatography - Mass Spectophotometry (LC-MS) Analysis: This product was subjected to LC-MS.

Applications

Key Applications

Western Blotting

Application Notes

Liquid Chromatography - Mass Spectophotometry (LC-MS) Analysis: This product was subjected to LC-MS.

Biological Information
Molecular Weight	~17 and 34 kDa observed

Biological Information

Molecular Weight

~17 and 34 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting using a panel of polyubiquitin proteins. Western Blotting Analysis: 0.14 µg/µL of Cat. No. MABS199, Anti-linear Ubiquitin, clone 1E3 detected Di-Ubiquitin (Linear) Recombinant Protein in a panel of polyubiquitin proteins.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting using a panel of polyubiquitin proteins.

Western Blotting Analysis: 0.14 µg/µL of Cat. No. MABS199, Anti-linear Ubiquitin, clone 1E3 detected Di-Ubiquitin (Linear) Recombinant Protein in a panel of polyubiquitin proteins.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store reconstituted solution at −80°C. Please avoid multiple freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Store reconstituted solution at −80°C. Please avoid multiple freeze/thaw cycles.

Packaging Information
Material Size	50 µg

Packaging Information

Material Size

50 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

GRB2, agarose conjugate

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

重要规格表

我要咨询