Anti-Red Fluorescent Protein, clone 1DS-1A6 (mouse monoclonal) - 炼石商城

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Anti-Red Fluorescent Protein, clone 1DS-1A6 (mouse monoclonal)

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货号：MAB10765

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MAB10765
Description	Anti-Red Fluorescent Protein Antibody, clone 1DS-1A6 \| MAB10765
Alternate Names	Red fluorescent protein
Background Information	Since the development of Green fluorescent protein’s (GFP) use as a protein tag in chimeric proteins, fluorescent proteins have become widely used and useful biotechnological tools. The isolation of Red Fluorescent proteins have opened up a broader color spectrum for use in research. These proteins can be fused with a wide variety of proteins at either the N- or C-terminus, and have been expressed as fluorescent markers in most cell types. These markers can be used as noninvasive markers in both living organisms and cells.

References

Product Information
Format	Ascites
Control	Cos cell lysate with overexpression of RFP
Presentation	Unpurified mouse monoclonal ascites with 0.05% sodium azide.

Applications
Key Applications	Western Blotting

Biological Information
Immunogen	Recombinant protein corresponding to sea anenome Red fluorescent protein.
Epitope	Unknown
Clone	1DS-1A6
Host	Mouse
Specificity	This antibody recognizes Red fluorescent protein.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Gene Symbol	RFP
Purification Method	Unpurified
UniProt Number	Q9U6Y8
UniProt Summary	FUNCTION: Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. SUBUNIT STRUCTURE: Homotetramer. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-N+2, oxidation of Tyr-N+1 to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGICAL USE: Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. SEQUENCE SIMILARITIES: Belongs to the GFP family. BIOPHYSICOCHEMICAL PROPERTIES: Absorption: Exhibits a smaller absorbance peak at 494 nm. The broad fluorescence emission spectrum peaks at 583 nm. Abs(max)=558 nm
Molecular Weight	~ 28 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in Cos cell lysate with overexpression of RFP. Western Blot Analysis: 1:1,000 dilution of this antibody detected Red fluorescent protein on 10 µg of Cos cell lysate with overexpression of RFP.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

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Supplemental Information

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