Anti-Ghrelin, clone 1ML-1D7 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	WB, ICC	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H

WB, ICC

Ascites

Monoclonal Antibody

Description
Catalogue Number	MAB10404
Description	Anti-Ghrelin Antibody, clone 1ML-1D7
Alternate Names	ghrelin/obestatin prepropeptide1 ghrelin/obestatin2 ghrelin, growth hormone secretagogue receptor ligand1 obestatin1 ghrelin/obestatin preprohormone Motilin-related peptide2 ghrelin1 M46 protein3 Growth hormone secretagogue
Background Information	Ghrelin is a 28 amino acids peptide with an essential n-octonayl modification at Serine 3. This peptide hormone is known as an endogenous ligand for the growth hormone secretagogue receptor and shares sequence similarity with the peptide hormone Motilin. Ghrelin displays pleotropic actions including growth hormone secretion, feeding behaviour and gastrointestinal motility. It is predominantly produced by enteroendocrine cells from the gastrointestinal tract, but has been shown to play a significant role in neurotrophy, particularly in the hippocampus, and appears to be essential for cognitive adaptation to changing environments and the process of learning.

Description

Catalogue Number

MAB10404

Description

Anti-Ghrelin Antibody, clone 1ML-1D7

Alternate Names

ghrelin/obestatin prepropeptide1
ghrelin/obestatin2
ghrelin, growth hormone secretagogue receptor ligand1
obestatin1
ghrelin/obestatin preprohormone
Motilin-related peptide2
ghrelin1
M46 protein3
Growth hormone secretagogue

Background Information

Ghrelin is a 28 amino acids peptide with an essential n-octonayl modification at Serine 3. This peptide hormone is known as an endogenous ligand for the growth hormone secretagogue receptor and shares sequence similarity with the peptide hormone Motilin. Ghrelin displays pleotropic actions including growth hormone secretion, feeding behaviour and gastrointestinal motility. It is predominantly produced by enteroendocrine cells from the gastrointestinal tract, but has been shown to play a significant role in neurotrophy, particularly in the hippocampus, and appears to be essential for cognitive adaptation to changing environments and the process of learning.

Product Information
Format	Ascites
Control	Mouse stomach tissue lysate
Presentation	Unpurified mouse monoclonal ascites with 0.05% sodium azide.

Product Information

Format

Ascites

Control

Mouse stomach tissue lysate

Presentation

Unpurified mouse monoclonal ascites with 0.05% sodium azide.

Applications
Application	Detect Ghrelin using this Anti-Ghrelin Antibody, clone 1ML-1D7 validated for use in WB & IC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ghrelin in A431 cells.

Applications

Application

Detect Ghrelin using this Anti-Ghrelin Antibody, clone 1ML-1D7 validated for use in WB & IC.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ghrelin in A431 cells.

Biological Information
Immunogen	Linear Peptide corresponding to the N-terminus of human Ghrelin.
Epitope	N-terminus
Clone	clone 1ML-1D7
Host	Mouse
Specificity	This antibody recognizes Ghrelin at the N-terminus.
Isotype	IgG1κ
Species Reactivity	Mouse Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_057446.1
Entrez Gene Summary	This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq].
Gene Symbol	MTLRP1 GHRL
Purification Method	Unpurified
UniProt Number	Q9UBU3
UniProt Summary	FUNCTION: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility By similarity. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid. Amidation of Leu-98 is essential for obestatin activity by similarity. SEQUENCE SIMILARITIES: Belongs to the motilin family. MASS SPECTROMETRY: Molecular mass is 3398.9±0.3 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10, O-decanoylated form. Molecular mass is 3397.2±0.5 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10:1, O-decenoylated form. Molecular mass is 3371.3±0.1 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C8, O-octanoylated form. Molecular mass is 3243.6±0.4 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C10, O-decanoylated form. Molecular mass is 3214.6±0.6 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C8, O-octanoylated form.
Molecular Weight	13 kDa calculated and observed

Biological Information

Immunogen

Linear Peptide corresponding to the N-terminus of human Ghrelin.

Epitope

N-terminus

Clone

clone 1ML-1D7

Host

Mouse

Specificity

This antibody recognizes Ghrelin at the N-terminus.

Isotype

IgG1κ

Species Reactivity

Mouse
Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_057446.1

Entrez Gene Summary

This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq].

Gene Symbol

MTLRP1
GHRL

Purification Method

Unpurified

UniProt Number

Q9UBU3

UniProt Summary

FUNCTION: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility By similarity.

SUBCELLULAR LOCATION: Secreted.

TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.

Amidation of Leu-98 is essential for obestatin activity by similarity.

SEQUENCE SIMILARITIES: Belongs to the motilin family.

MASS SPECTROMETRY: Molecular mass is 3398.9±0.3 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10, O-decanoylated form.

Molecular mass is 3397.2±0.5 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10:1, O-decenoylated form.

Molecular mass is 3371.3±0.1 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C8, O-octanoylated form.

Molecular mass is 3243.6±0.4 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C10, O-decanoylated form.

Molecular mass is 3214.6±0.6 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C8, O-octanoylated form.

Molecular Weight

13 kDa calculated and observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse stomach tissue lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected Ghrelin in 10 µg of mouse stomach tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in mouse stomach tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Ghrelin in 10 µg of mouse stomach tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Ghrelin, clone 1ML-1D7 (mouse monoclonal)

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