Anti-Amphiphysin (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABT21
Description	Anti-Amphiphysin II Antibody
Alternate Names	bridging integrator 1 amphiphysin-like box dependant MYC interacting protein 1 Amphiphysin II Box-dependent myc-interacting protein 1 Amphiphysin-like protein
Background Information	Amphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes.

Description

Catalogue Number

ABT21

Description

Anti-Amphiphysin II Antibody

Alternate Names

bridging integrator 1
amphiphysin-like
box dependant MYC interacting protein 1
Amphiphysin II
Box-dependent myc-interacting protein 1
Amphiphysin-like protein

Background Information

Amphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes.

Product Information
Format	Affinity Purified
Control	Mouse brain tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Mouse brain tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates.

Applications

Application

Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates.

Biological Information
Immunogen	KLH-conjugated recombinant protein corresponding to human Amphiphysin II.
Epitope	Unknown
Host	Rabbit
Specificity	The antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes).
Isotype	IgG
Species Reactivity	Mouse Rat
Species Reactivity Note	Demonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004296
Entrez Gene Summary	This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq].
Gene Symbol	AMPHL AMPH2 BIN1 SH3P9
Purification Method	Affinity Purfied
UniProt Number	O00499
UniProt Summary	FUNCTION: May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. SUBUNIT STRUCTURE: Heterodimer with AMPH. Binds SH3GLB1 By similarity. Interacts (via SH3 domain) with SYNJ1. Interacts (via SH3 domain) with DNM1. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1. Interacts with MYC (via N-terminal transactivation domain); the interaction requires of in a the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts (SH3 domain) with HCV NS5A. SUBCELLULAR LOCATION: Isoform BIN1: Nucleus. Isoform IIA: Cytoplasm. TISSUE SPECIFICTY: Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. PTM: Phosphorylated by protein kinase C By similarity. INVOLVEMENT IN DISEASE: Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. SEQUENCE SIMILARITIES: Contains 1 BAR domain. Contains 1 SH3 domain.
Molecular Weight	It is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights.

Biological Information

Immunogen

KLH-conjugated recombinant protein corresponding to human Amphiphysin II.

Epitope

Unknown

Host

Rabbit

Specificity

The antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes).

Isotype

IgG

Species Reactivity

Mouse
Rat

Species Reactivity Note

Demonstrated to react with mouse and rat. Predicted to react with human based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004296

Entrez Gene Summary

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq].

Gene Symbol

AMPHL
AMPH2
BIN1
SH3P9

Purification Method

Affinity Purfied

UniProt Number

O00499

UniProt Summary

FUNCTION: May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

SUBUNIT STRUCTURE: Heterodimer with AMPH. Binds SH3GLB1 By similarity. Interacts (via SH3 domain) with SYNJ1. Interacts (via SH3 domain) with DNM1. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1. Interacts with MYC (via N-terminal transactivation domain); the interaction requires of in a the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts (SH3 domain) with HCV NS5A.

SUBCELLULAR LOCATION: Isoform BIN1: Nucleus.

Isoform IIA: Cytoplasm.

TISSUE SPECIFICTY: Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

PTM: Phosphorylated by protein kinase C By similarity.

INVOLVEMENT IN DISEASE: Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.

SEQUENCE SIMILARITIES: Contains 1 BAR domain.

Contains 1 SH3 domain.

Molecular Weight

It is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Amphiphysin (rabbit polyclonal)

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