Anti-Rac1 (rabbit polyclonal)

Replacement Information
Replacement Information	07-1464 is a recommended replacement for AB4202

Replacement Information

07-1464 is a recommended replacement for AB4202

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Purified

Polyclonal Antibody

Description
Catalogue Number	07-1464
Replaces	AB4202
Description	Anti-Rac1 Antibody
Alternate Names	Ras-related C3 Botulinum Toxin Substrate 1 p21-Rac1 Ras-like Protein TC25
Background Information	Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Description

Catalogue Number

07-1464

Replaces

AB4202

Description

Anti-Rac1 Antibody

Alternate Names

Ras-related C3 Botulinum Toxin Substrate 1
p21-Rac1
Ras-like Protein TC25

Background Information

Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Product Information
Format	Purified
Control	Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation Immunohistochemistry: Rat brain sections
Presentation	Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative

Product Information

Format

Purified

Control

Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections

Presentation

Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative

Applications
Application	Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.
Key Applications	Western Blotting
Application Notes	Optimal working dilutions must be determined by end user.

Applications

Application

Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.

Key Applications

Western Blotting

Application Notes

Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_006908.4
Entrez Gene Summary	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
Gene Symbol	RAC1 ORFNames=MIG5 TC-25 p21-Rac1 MGC111543 PAK1 MIG5
Purification Method	Protein A Purfied
UniProt Number	P63000
UniProt Summary	FUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. FUNCTION: Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH (By similarity). SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
Molecular Weight	21 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_006908.4

Entrez Gene Summary

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Gene Symbol

RAC1
ORFNames=MIG5
TC-25
p21-Rac1
MGC111543
PAK1
MIG5

Purification Method

Protein A Purfied

UniProt Number

P63000

UniProt Summary

FUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
FUNCTION: Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Molecular Weight

21 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on L6 lysates. Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Rac1 (rabbit polyclonal)

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