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Anti-TPO (rabbit polyclonal)

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货号：ABS682

品牌：Millipore 密理博

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Replacement Information

特卖

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R ICC Rb AlexaFluor®647 Polyclonal Antibody
Description
Catalogue Number ABS82-AF647
Description Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate
Alternate Names
  • Vascular endothelial growth factor A
  • VEGF-A
  • Vascular permeability factor
  • VPF
  • VEGF, Alexa Fluor® 647 Conjugate
Background Information VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
References
Product Information
Format AlexaFluor®647
Presentation Purified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Applications
Key Applications
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.
Epitope N-terminus
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity This antibody recognizes VEGF at the N-terminus.
Isotype IgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Human, Mouse, Rat. Predicted to react with Non-Human Primate based on 100% sequence homology. Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology). Predicted to react with primate based on 100% sequence homology..
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VEGFA
  • VEGF
Purification Method Affinity Purfied
UniProt Number
Molecular Weight ~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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