Anti-GIT1 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Chp	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, Chp

WB, IH(P)

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABT34
Description	Anti-Podoplanin Antibody
Alternate Names	Podoplanin Aggrus Glycoprotein 36 Gp36 PA2.26 antigen T1-alpha T1A
Background Information	Podoplanin, also known as T1 alpha and Aggrus, is a mucin-type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. Podoplanin regulates lymphatic vascular formation and platelet aggregation and is thought to be involved in cell migration and/or actin cytoskeleton organization. Podoplanin expression in keratinocytes induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility, and decreased cell adhesion. Podoplanin does not have any effect on folic acid or amino acid transport nor does it function as a water channel or as a regulator of aquaporin-type water channels.

Description

Catalogue Number

ABT34

Description

Anti-Podoplanin Antibody

Alternate Names

Podoplanin
Aggrus
Glycoprotein 36
Gp36
PA2.26 antigen
T1-alpha
T1A

Background Information

Podoplanin, also known as T1 alpha and Aggrus, is a mucin-type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. Podoplanin regulates lymphatic vascular formation and platelet aggregation and is thought to be involved in cell migration and/or actin cytoskeleton organization. Podoplanin expression in keratinocytes induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility, and decreased cell adhesion. Podoplanin does not have any effect on folic acid or amino acid transport nor does it function as a water channel or as a regulator of aquaporin-type water channels.

Product Information
Format	Affinity Purified
Control	Human lung tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Human lung tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-Podoplanin Antibody detects level of Podoplanin & has been published & validated for use in WB, IH(P).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in colorectal carcinoma tissue.

Applications

Application

Anti-Podoplanin Antibody detects level of Podoplanin & has been published & validated for use in WB, IH(P).

Key Applications

Western Blotting
Immunohistochemistry (Paraffin)

Application Notes

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in colorectal carcinoma tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Podoplanin at the extracellular domain.
Epitope	Extracellular domain
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Podoplanin at the extracellular domain.
Species Reactivity	Human Mouse Chimpanzee
Species Reactivity Note	Demonstrated to react with Human and Mouse. Predicted to react with Chimpanzee based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006465
Entrez Gene Summary	This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Gene Symbol	PDPN GP36
Purification Method	Affinity Purfied
UniProt Number	Q86YL7
UniProt Summary	FUNCTION: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (By similarity). Cell projection › filopodium membrane; Single-pass type I membrane protein (By similarity). Cell projection › lamellipodium membrane; Single-pass type I membrane protein (By similarity). Cell projection › microvillus membrane; Single-pass type I membrane protein (By similarity). Cell projection › ruffle membrane; Single-pass type I membrane protein (By similarity). Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles (By similarity). TISSUE SPECIFICITY: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. PTM: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. The N-terminus is blocked (By similarity). SEQUENCE SIMILARITIES: Belongs to the podoplanin family. SEQUENCE CAUTION: The sequence AAM73655.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAO22143.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC11550.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC11557.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG35495.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight	~27 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing with molecular weights of ~17, ~18, and two at ~25 kDa. The calculated molecular weight of this protein is 25 kDa, but can be observed at ~27 kDa due to high glycosylation.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human Podoplanin at the extracellular domain.

Epitope

Extracellular domain

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes Podoplanin at the extracellular domain.

Species Reactivity

Human
Mouse
Chimpanzee

Species Reactivity Note

Demonstrated to react with Human and Mouse. Predicted to react with Chimpanzee based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006465

Entrez Gene Summary

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Gene Symbol

PDPN
GP36

Purification Method

Affinity Purfied

UniProt Number

Q86YL7

UniProt Summary

FUNCTION: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (By similarity). Cell projection › filopodium membrane; Single-pass type I membrane protein (By similarity). Cell projection › lamellipodium membrane; Single-pass type I membrane protein (By similarity). Cell projection › microvillus membrane; Single-pass type I membrane protein (By similarity). Cell projection › ruffle membrane; Single-pass type I membrane protein (By similarity). Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles (By similarity).

TISSUE SPECIFICITY: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

PTM: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.

The N-terminus is blocked (By similarity).

SEQUENCE SIMILARITIES: Belongs to the podoplanin family.

SEQUENCE CAUTION: The sequence AAM73655.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAO22143.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC11550.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC11557.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAG35495.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Molecular Weight

~27 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing with molecular weights of ~17, ~18, and two at ~25 kDa. The calculated molecular weight of this protein is 25 kDa, but can be observed at ~27 kDa due to high glycosylation.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human lung tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected Podoplanin on 10 µg of human lung tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Podoplanin on 10 µg of human lung tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-GIT1 (rabbit polyclonal)

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