Anti-CLINT1 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Purified

Polyclonal Antibody

Description
Catalogue Number	ABS71
Description	Anti-GEF-H1 Antibody
Alternate Names	Rho/Rac guanine nucleotide exchange factor (GEF) 2 Rho/Rac guanine nucleotide exchange factor 2 Proliferating cell nucleolar antigen p40 Microtubule-regulated Rho-GEF Guanine nucleotide exchange factor H1
Background Information	Guanine nucleotide exchange factor H1 (GEF-H1) is a guanine nucleotide exchange factor containing 3 characteristic domains; a DH (DBL-homology) domain that functions to interact with and promote GTP loading on RhoA, a pleckstrin-homology (PH) domain that participates in the binding and targeting of microtubules to tight junctions, and a phorbol-ester/DAG-type zinc finger. GEF-H1 functions to promote the GDP to GTP exchange for the activation of Rho-GTPases. Various studies have suggested that GEF-H1 may have several diverse roles such as; dendritic spine motility, cell motility and polarization, cell cycle regulation, antigen presentation, epithelial barrier permeability, leukemic cell differentiation, cancer, and accelerated cell proliferation within tumor cells. Currently, there are three known isoforms resulting from alternative splicing.

Description

Catalogue Number

ABS71

Description

Anti-GEF-H1 Antibody

Alternate Names

Rho/Rac guanine nucleotide exchange factor (GEF) 2
Rho/Rac guanine nucleotide exchange factor 2
Proliferating cell nucleolar antigen p40
Microtubule-regulated Rho-GEF
Guanine nucleotide exchange factor H1

Background Information

Guanine nucleotide exchange factor H1 (GEF-H1) is a guanine nucleotide exchange factor containing 3 characteristic domains; a DH (DBL-homology) domain that functions to interact with and promote GTP loading on RhoA, a pleckstrin-homology (PH) domain that participates in the binding and targeting of microtubules to tight junctions, and a phorbol-ester/DAG-type zinc finger. GEF-H1 functions to promote the GDP to GTP exchange for the activation of Rho-GTPases. Various studies have suggested that GEF-H1 may have several diverse roles such as; dendritic spine motility, cell motility and polarization, cell cycle regulation, antigen presentation, epithelial barrier permeability, leukemic cell differentiation, cancer, and accelerated cell proliferation within tumor cells. Currently, there are three known isoforms resulting from alternative splicing.

Product Information
Format	Purified
Control	HEK293 cell lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

HEK293 cell lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-GEF-H1 Antibody is an antibody against GEF-H1 for use in WB.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: 2 µg/mL of this antibody was shown to detect GEF-H1 on 10 µg of L6 cell lysate.

Applications

Application

Anti-GEF-H1 Antibody is an antibody against GEF-H1 for use in WB.

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: 2 µg/mL of this antibody was shown to detect GEF-H1 on 10 µg of L6 cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human GEF-H1.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes GEF-H1.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004714
Entrez Gene Summary	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq].
Gene Symbol	GEF GEF-H1 LFP40 P40 GEFH1
Purification Method	Protein G Purified
UniProt Number	Q92974
UniProt Summary	FUNCTION: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in Ref.1 (Ren Y., et al. (1998). J. Biol. Chem. 273:34954-34960.). May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. SUBUNIT STRUCTURE: Interacts with 14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA/STK6 and MAPK1. Interacts with RHOA and RAC1. SUBCELLULAR LOCATION: Cytoplasm. Cell junction › tight junction (By similarity). Golgi apparatus. Cytoplasm › cytoskeleton › spindle. Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. DOMAIN: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions. PTM: Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. SEQUENCE SIMLARITIES: Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. SEQUENCE CAUTION: The sequence AAC97383.1 differs from that shown. Reason: Sequence differs at a large extent from the sequence shown in the paper. The sequence AAH20567.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA33634.1 differs from that shown. Reason: Frameshift at positions 887 and 984.
Molecular Weight	~ 120 kDa observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human GEF-H1.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes GEF-H1.

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Demonstrated to react with human, mouse, and rat.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004714

Entrez Gene Summary

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq].

Gene Symbol

GEF
GEF-H1
LFP40
P40
GEFH1

Purification Method

Protein G Purified

UniProt Number

Q92974

UniProt Summary

FUNCTION: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in Ref.1 (Ren Y., et al. (1998). J. Biol. Chem. 273:34954-34960.). May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases.

SUBUNIT STRUCTURE: Interacts with 14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA/STK6 and MAPK1. Interacts with RHOA and RAC1.

SUBCELLULAR LOCATION: Cytoplasm. Cell junction › tight junction (By similarity). Golgi apparatus. Cytoplasm › cytoskeleton › spindle. Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization.

DOMAIN: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.

The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.

PTM: Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.

SEQUENCE SIMLARITIES: Contains 1 DH (DBL-homology) domain.

Contains 1 PH domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

SEQUENCE CAUTION: The sequence AAC97383.1 differs from that shown. Reason: Sequence differs at a large extent from the sequence shown in the paper.

The sequence AAH20567.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA33634.1 differs from that shown. Reason: Frameshift at positions 887 and 984.

Molecular Weight

~ 120 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected GEF-H1 on 10 µg of HEK293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected GEF-H1 on 10 µg of HEK293 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CLINT1 (rabbit polyclonal)

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