Anti-Interleukin 23 alpha (IL-23A)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, FC	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, FC

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	06-1079
Description	Anti-Interleukin 23 alpha (IL-23A) Antibody
Alternate Names	IL-23 subunit alpha Interleukin-23 subunit p19 JKA3 induced upon T-cell activation interleukin 23 p19 subunit interleukin 23, alpha subunit p19
Background Information	Interleukin-23 subunit alpha (IL-23A or IL-23, p19 subunit) is a subunit of heterodimeric cytokine IL-23, which also contains the p40 subunit of interleukin 12 (IL-12B). The receptor of IL-23 is formed by the beta 1 subunit of IL-12 (IL-12RB1) and an IL-23 specific subunit, IL-23R. IL-23 activates the transcription activator STAT4, and stimulates the production of interferon-gamma. IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. In conjunction with IL-6 and TGF-β1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation.

Description

Catalogue Number

06-1079

Description

Anti-Interleukin 23 alpha (IL-23A) Antibody

Alternate Names

IL-23 subunit alpha
Interleukin-23 subunit p19
JKA3 induced upon T-cell activation
interleukin 23 p19 subunit
interleukin 23, alpha subunit p19

Background Information

Interleukin-23 subunit alpha (IL-23A or IL-23, p19 subunit) is a subunit of heterodimeric cytokine IL-23, which also contains the p40 subunit of interleukin 12 (IL-12B). The receptor of IL-23 is formed by the beta 1 subunit of IL-12 (IL-12RB1) and an IL-23 specific subunit, IL-23R. IL-23 activates the transcription activator STAT4, and stimulates the production of interferon-gamma. IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. In conjunction with IL-6 and TGF-β1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation.

Product Information
Format	Affinity Purified
Control	3T3/A31 cell lysate
Presentation	Purified rabbit polyclonal in PBS with 0.09% sodium azide.

Product Information

Format

Affinity Purified

Control

3T3/A31 cell lysate

Presentation

Purified rabbit polyclonal in PBS with 0.09% sodium azide.

Applications
Application	Detect Interleukin 23 alpha (IL-23A) using this Anti-Interleukin 23 alpha (IL-23A) Antibody validated for use in WB, FC.
Key Applications	Western Blotting Flow Cytometry
Application Notes	Flow Cytometry Analysis: 1 µg from a previous lot detected IL-23 alpha in human PBMC's.

Applications

Application

Detect Interleukin 23 alpha (IL-23A) using this Anti-Interleukin 23 alpha (IL-23A) Antibody validated for use in WB, FC.

Key Applications

Western Blotting
Flow Cytometry

Application Notes

Flow Cytometry Analysis: 1 µg from a previous lot detected IL-23 alpha in human PBMC's.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the mature chain of human IL-23 subunit alpha.
Epitope	Mature chain of IL-23, subunit alpha.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Antibody recognizes the mature chain of IL-23 subunit alpha.
Species Reactivity	Human Mouse
Species Reactivity Note	Demonstrated to react with human and mouse. Predicted to react with rhesus monkey based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_057668
Entrez Gene Summary	This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq].
Gene Symbol	IL23A IL-23 IL-23A IL-23p19 IL23P19 P19 SGRF
Purification Method	Affinity Purfied
UniProt Number	Q9NPF7
UniProt Summary	FUNCTION: Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. SUBUNIT STRUCTURE: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-23. SUBCELLULAR LOCATION: Secreted. Note: Secreted upon association with IL12B. TISSUE SPECIFICTY: Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). DEVELOPMENTAL STAGE: Expressed by newborns dendritic cells. INDUCTION: Up-regulated by a wide array of pathogens and pathogen-products together with self-signals for danger or injury. Up-regulated in psoriatic dermal tissues, in dendritic cells of multiple sclerosis patients and in tumors. SEQUENCE SIMILARITIES: Belongs to the IL-6 superfamily.
Molecular Weight	UniProtKB/Swiss-Prot entry Q9NPF7 lists a molecular weight of 20.730 kDa. In some lysates tested, non-specific bands are observed at around 40 kDa and around 55 kDa.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to the mature chain of human IL-23 subunit alpha.

Epitope

Mature chain of IL-23, subunit alpha.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Antibody recognizes the mature chain of IL-23 subunit alpha.

Species Reactivity

Human
Mouse

Species Reactivity Note

Demonstrated to react with human and mouse.
Predicted to react with rhesus monkey based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_057668

Entrez Gene Summary

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq].

Gene Symbol

IL23A
IL-23
IL-23A
IL-23p19
IL23P19
P19
SGRF

Purification Method

Affinity Purfied

UniProt Number

Q9NPF7

UniProt Summary

FUNCTION: Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

SUBUNIT STRUCTURE: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-23.

SUBCELLULAR LOCATION: Secreted. Note: Secreted upon association with IL12B.

TISSUE SPECIFICTY: Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

DEVELOPMENTAL STAGE: Expressed by newborns dendritic cells.

INDUCTION: Up-regulated by a wide array of pathogens and pathogen-products together with self-signals for danger or injury. Up-regulated in psoriatic dermal tissues, in dendritic cells of multiple sclerosis patients and in tumors.

SEQUENCE SIMILARITIES: Belongs to the IL-6 superfamily.

Molecular Weight

UniProtKB/Swiss-Prot entry Q9NPF7 lists a molecular weight of 20.730 kDa. In some lysates tested, non-specific bands are observed at around 40 kDa and around 55 kDa.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in 3T3/A31 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected IL-23 alpha in 10 µg of 3T3/A31 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in 3T3/A31 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-23 alpha in 10 µg of 3T3/A31 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-Interleukin 23 alpha (IL-23A)

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