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Anti-ALMS1 (rabbit polyclonal)

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货号：ABT414

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, B WB, FC, IP, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MABT414
Description Anti-Moesin Antibody, clone 38/87
Alternate Names
  • Moesin
  • Membrane-organizing extension spike protein
Background Information Moesin (membrane-organizing extension spike protein) is the protein encoded by the MSN gene. Moesin is a member of the ERM (ezrin, moesin, radixin) family of proteins that regulate linkage between the plasma membrane and the actin cytoskeleton. Thus, moesin is a peripheral membrane protein that is keenly involved in the signaling and structural connections in cytoskeletal plasma membrane structures like microvilli. Moesin in its active, phosphorylated state strongly binds actin and helps anchor it to the plasma membrane. Moesin is localized to filopodia and other membranous extensions of various cell types and thus plays a role in cell-cell recognition signaling, cell movement, and cell transport. In its inactive, non-phosphorylated state, Moesin binds itself in a head-tail fashion and is incapable of binding actin or other cytoskeletal proteins. The kinase ROCK2 is central to the opening up of moesin and allowing its interaction with actin and the plasma membrane.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Detect Moesin using this mouse monoclonal antibody, Anti-Moesin Antibody, clone 38/87 validated for use in western blotting, Flow Cytometry, IP & IHC.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry
Application Notes Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Moesin in 10 µg of HL-60 cell lysate.

Flow Cytometry Analysis: 4 µg from a representative lot detected Moesin in 1X10E6 HeLa cells.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected Moesin in THP-1, U937, and MOLT-4 cells (Takamatsu, H., et al. (2009). J Immunol. 182(1):703-710.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Moesin in purified heparain-binding proteins, and from human renal cortex tisssue (Lankes, W., et al. (1988). Biochem J. 251(3):831-842.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Moesin in HT-29 cell lysate (Lankes, W., et al. (1988). Biochem J. 251(3):831-842.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Moesin in certain human brain tissues (Johnson, M. W., et al. (2002). Acta Neuropathol. 104(2):188-196.).
Biological Information
Immunogen Recombinant protein corresponding to bovine Moesin.
Clone 38/87
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
  • Bovine
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MSN
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~78 kDa observed. This protein has been observed at ~78 kDa due to post-translational modification (Lankes, W., et al. (1988). Biochem J. 251(3):831-842.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Moesin in 10 µg of HL-60 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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