ANTI-EMT/ITK/TSK PC 12-303

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ANTI-EMT/ITK/TSK PC 12-303

市场价：: 0.00

促销价：: ¥0.00

货号：06-546

品牌：Millipore 密理博

配送至：

北京朝阳

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数量：

EA (预计3-5工作日到货)

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Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	06-1064
Replaces	06-546
Description	Anti-ITK/EMT/TSK Antibody
Alternate Names	IL2-inducible T-cell kinase Kinase EMT T-cell-specific kinase Tyrosine-protein kinase Lyk homolog of mouse T-cell itk/tsk tyrosine-protein kinase ITK/TSK
Background Information	Tyrosine-protein kinase ITK/TSK (UniProt: Q08881; also known as EC:2.7.10.2, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LYK) gene (Gene ID: 3702) in human. ITK/TSK is a protein tyrosine kinase that plays an essential role in regulation of the adaptive immune response. It regulates the development, function, and differentiation of conventional T-cells and non-conventional natural killer T-cells. It contains four zinc binding sites. It s N-terminal PH domain allows it to be recruited to the plasma membrane by an activated PI3 kinase. This domain also contains a proline-rich region (PRR). The adjoining SH3 domain binds to PRR. The SH2 domain is required for binding tyrosine-phosphorylated substrates and the kinase domain located in the C-terminal region is required for tyrosine phosphorylation. ITK/TSK is phosphorylated at Tyr512 in the activation loop of the kinase domain by LCK and subsequent autophosphorylation at Tyr180, which lies within the substrate-binding sequence in the SH3 domain, allows the kinase activation. Mutations in ITK gene are reported to cause lymphoproliferative syndrome 1, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus that can lead to fatal outcome.

References

Product Information
Format	Affinity Purified
Control	Jurkat cell lysates
Presentation	Purified rabbit polycloncal in buffer containing PBS with 0.05% sodium azide and 50% glycerol.

Applications
Application	This rabbit polyclonal Anti-ITK/EMT/TSK, Cat. No. 06-1064 is a rabbit polyclonal antibody that targets protein tyrosine kinase ITK/TSK and is tested for use in Immunoprecipitation and Western blotting.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated ITK/EMT/TSK in 500 µg of Jurkat cell lysate.

Biological Information
Immunogen	GST-tagged recombinant fragment cvorresponding to the first 270 amino acids from the N-terminal half of human Tyrosine-protein kinase ITK/TSK spanning the PH, SH3 and a small portion of the SH2 domains.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Itk/Emt/Tsk, Mr ~72 kDa.
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to cross-react with mouse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005537
Gene Symbol	ITK EMT LYK
Purification Method	Affinity Purfied
UniProt Number	Q08881
Molecular Weight	~71 kDa observed; 71.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected ITK/EMT/TSK in 10 µg of Jurkat cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.