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Anti-Myc Tag

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货号：06-549

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ICC, WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 06-549
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Myc Tag Antibody
Alternate Names
  • Transcription factor p64
  • avian myelocytomatosis viral oncogene homolog
  • myc proto-oncogene protein
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
Background Information Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
References
Product Information
Format Purified
Control
  • Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Presentation Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
Applications
Application Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application Notes Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.
Biological Information
Immunogen Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).
Epitope a.a. 409-420
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Isotype IgG
Species Reactivity
  • Human
Species Reactivity Note Human. Other species cross-reactivity not tested.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
Gene Symbol
  • MYC
  • C-MYC
  • c-Myc
Purification Method Protein A chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Molecular Weight Predicted molecular weight for human Myc: 49 kDa. Obeserved molecular weight may vary.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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