Anti-Gab1, CT (Rabbit Polyclonal)

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准

Anti-Gab1, CT (Rabbit Polyclonal)

市场价：: 0.00

促销价：: ¥0.00

货号：06-579

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

10ul吸头，透明，袋装，可灭菌...

85.80

UltraSignal 超敏ECL化学发光...

686.00

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, IH(P), IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	06-579
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Gab1 Antibody, CT
Alternate Names	GRB2-associated binder GRB2-associated binding protein Growth factor receptor bound protein 2-associated protein
Background Information	Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFR-β]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

References

Product Information
Format	Affinity Purified
Control	Non-stimulated A431 carcinoma cell lysate or NIH/3T3 cytoplasmic lysate.
Presentation	Affinity purified IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

Applications
Application	Anti-Gab1 Antibody, CT detects level of Gab1 & has been published & validated for use in IC, IH(P), IP & WB.
Key Applications	Immunocytochemistry Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: 4 μg of this lot immunoprecipitated Gab1 from 500 μg of a human A431 carcinoma cell RIPA lysate. Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Gab1 in A431 cells fixed with 4% paraformaldehyde.

Biological Information
Immunogen	31 residue peptide sequence corresponding to C-terminal residues 664-694 of Gab1 (CQKTLALKSTREAWTDGRQSTESETPAKSVK).
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Specific for Gab1. No other known protein shares more than 10 amino acids with the immunizing sequence.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, mouse and rat. Reactivity with other species has not been confirmed.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_207123
Entrez Gene Summary	The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	GAB1
Purification Method	Affinity Purfied
UniProt Number	Q13480
UniProt Summary	FUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling. SIZE: 694 amino acids; 76616 Da SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain.
Molecular Weight	115 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on RIPA lysates from human A431 carcinoma cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Gab1 in RIPA lysates from human A431 carcinoma cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.