Anti-phospho-p38a (Thr180/Tyr182)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

IP, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-272
Replaces	AB3828
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-p38α (Thr180/Tyr182) Antibody
Alternate Names	SAPK2a MAPK14 CSBP1 CSBP2 CSPB1 PRKM14 PRKM15 p38ALPHA mitogen-activated protein kinase 14

Description

Catalogue Number

09-272

Replaces

AB3828

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-p38α (Thr180/Tyr182) Antibody

Alternate Names

SAPK2a
MAPK14
CSBP1
CSBP2
CSPB1
PRKM14
PRKM15
p38ALPHA
mitogen-activated protein kinase 14

Product Information
Format	Affinity Purified
Control	Anisomycin treated HeLa cells
Presentation	Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Anisomycin treated HeLa cells

Presentation

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Applications
Application	This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).
Key Applications	Immunoprecipitation Western Blotting
Application Notes	Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin. Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin. Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer. Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.

Applications

Application

This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).

Key Applications

Immunoprecipitation
Western Blotting

Application Notes

Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.

Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.

Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.

Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.

Biological Information
Immunogen	Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes p38α dual phosphorylation of Thr180 and Tyr182.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001315.1 NM_139012.1 NM_139013.1 NM_139014.1
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Gene Symbol	MAPK14 PRKM14 RK SAPK2A CSBP1 p38 CSPB1 CSBP PRKM15 Mxi2 p38ALPHA MXI2 EXIP CSBP2
Modifications	Phosphorylation
Purification Method	ImmunoAffinity Purified
UniProt Number	Q16539
UniProt Summary	FUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. COFACTOR: Magnesium. SIZE: 360 amino acids; 41293 Da SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight	38 kDa

Biological Information

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes p38α dual phosphorylation of Thr180 and Tyr182.

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

Entrez Gene Summary

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Gene Symbol

MAPK14
PRKM14
RK
SAPK2A
CSBP1
p38
CSPB1
CSBP
PRKM15
Mxi2
p38ALPHA
MXI2
EXIP
CSBP2

Modifications

Phosphorylation

Purification Method

ImmunoAffinity Purified

UniProt Number

Q16539

UniProt Summary

FUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
COFACTOR: Magnesium.
SIZE: 360 amino acids; 41293 Da
SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.

Molecular Weight

38 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunoblot.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-phospho-p38a (Thr180/Tyr182)

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