Anti-phospho-Chk2 (Thr68) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Po, Chp, Rhesus Macaque	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Po, Chp, Rhesus Macaque

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABE33
Description	Anti-phospho-Chk2 (Thr68) Antibody
Alternate Names	CHK2 (checkpoint, S.pombe) homolog CHK2 checkpoint homolog (S. pombe) checkpoint-like protein CHK2 protein kinase CHK2 serine/threonine-protein kinase CHK2
Background Information	Chk2 is a protein kinase activated and phosphorylated in response to DNA damage, especially DNA double-strand breaks. As a cell cycle checkpoint regulator it acts to prevent DNA replication and inhibit mitotic entry prior to the conclusion of DNA replication. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene mutated in breast and ovarian cancer. There are 12 known isoforms of Chk2. The majority of which are expressed solely in the cell nucleus. The exception is isoform 10 which is found throughout the cell. High expression has been observed in spleen, peripheral blood leukocytes, colon and testis. Lower expression is observed in other tissues. Mutations in CHEK2 gene coding for Chk2 are associated with Li-Fraumeni syndrome 2, and have been observed in some patients that contracted prostate cancer and osteosarcoma.

Description

Catalogue Number

ABE33

Description

Anti-phospho-Chk2 (Thr68) Antibody

Alternate Names

CHK2 (checkpoint, S.pombe) homolog
CHK2 checkpoint homolog (S. pombe)
checkpoint-like protein CHK2
protein kinase CHK2
serine/threonine-protein kinase CHK2

Background Information

Chk2 is a protein kinase activated and phosphorylated in response to DNA damage, especially DNA double-strand breaks. As a cell cycle checkpoint regulator it acts to prevent DNA replication and inhibit mitotic entry prior to the conclusion of DNA replication. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene mutated in breast and ovarian cancer.
There are 12 known isoforms of Chk2. The majority of which are expressed solely in the cell nucleus. The exception is isoform 10 which is found throughout the cell. High expression has been observed in spleen, peripheral blood leukocytes, colon and testis. Lower expression is observed in other tissues. Mutations in CHEK2 gene coding for Chk2 are associated with Li-Fraumeni syndrome 2, and have been observed in some patients that contracted prostate cancer and osteosarcoma.

Product Information
Format	Affinity Purified
Control	HeLa cell lysate
Presentation	Purified rabbit polyclonal containing PBS with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

HeLa cell lysate

Presentation

Purified rabbit polyclonal containing PBS with 0.05% sodium azide.

Applications
Application	Use the Anti-phospho-Chk2 Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Chk2 (Thr68) also known as CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2, protein kinase CHK2.
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: 1 µg/ml from a representative lot detected Chk2 on 10 µg of HEK293 cell lysate.

Applications

Application

Use the Anti-phospho-Chk2 Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Chk2 (Thr68) also known as CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2, protein kinase CHK2.

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: 1 µg/ml from a representative lot detected Chk2 on 10 µg of HEK293 cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Chk2 phosphorylated at Thr68.
Epitope	Thr68
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes Chk2 phosphorylated at Thr68.
Species Reactivity	Human Pig Chimpanzee Rhesus Macaque
Species Reactivity Note	Demonstrated to react with human. Predicted to react with pig, chimpanzee, and rhesus monkey based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_009125
Entrez Gene Summary	In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol	CHEK2 CDS1 CHK2 Cds1 HuCds1 LFS2 PP1425 RAD53
Purification Method	Affinity Purfied
UniProt Number	O96017
UniProt Summary	FUNCTION:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR:Magnesium. ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus. TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. PTM: Phosphorylated by PLK4. INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. Contains 1 FHA domain. Contains 1 protein kinase domain.
Molecular Weight	The observed molecular weight is 61 kDa. UniProtKB/Swiss-Prot O96017 (CHK2_HUMAN) describes 12 isoforms produced by alternative splicing.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human Chk2 phosphorylated at Thr68.

Epitope

Thr68

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes Chk2 phosphorylated at Thr68.

Species Reactivity

Human
Pig
Chimpanzee
Rhesus Macaque

Species Reactivity Note

Demonstrated to react with human. Predicted to react with pig, chimpanzee, and rhesus monkey based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_009125

Entrez Gene Summary

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene Symbol

CHEK2
CDS1
CHK2
Cds1
HuCds1
LFS2
PP1425
RAD53

Purification Method

Affinity Purfied

UniProt Number

O96017

UniProt Summary

FUNCTION:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

COFACTOR:Magnesium.

ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.

SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell.

Isoform 4: Nucleus.

Isoform 7: Nucleus.

Isoform 9: Nucleus.

Isoform 12: Nucleus.

TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

PTM: Phosphorylated by PLK4.

INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].

Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.

Contains 1 FHA domain.

Contains 1 protein kinase domain.

Molecular Weight

The observed molecular weight is 61 kDa. UniProtKB/Swiss-Prot O96017 (CHK2_HUMAN) describes 12 isoforms produced by alternative splicing.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected Chk2 on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Chk2 on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-Chk2 (Thr68) (rabbit polyclonal)

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