Rb X MaxiK

Species Reactivity	Key Applications	Host	Format	Antibody Type
R	IHC, IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

IHC, IP, WB

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	AB9770-200UL
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-MaxiK Antibody, aa1184-1200.
Alternate Names	K_CA1.1 BK_CA Large conductance Ca²⁺-activated K⁺ channel BKCa Maxi K⁺ KCNMA1

Description

Catalogue Number

AB9770-200UL

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-MaxiK Antibody, aa1184-1200.

Alternate Names

K_CA1.1
BK_CA
Large conductance Ca²⁺-activated K⁺ channel
BKCa
Maxi K⁺
KCNMA1

Product Information
Format	Affinity Purified
Control	Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation	Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Product Information

Format

Affinity Purified

Control

Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Presentation

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Applications
Application	Detect MaxiK using this Anti-MaxiK Antibody, aa1184-1200. validated for use in IH, IP & WB.
Key Applications	Immunohistochemistry Immunoprecipitation Western Blotting
Application Notes	Western blot: 1:500 using ECL on rat brain membranes. Immunohistochemistry: On rat brain sections. Immunoprecipitation: 4 μg using rat brain lysate. Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user.

Applications

Application

Detect MaxiK using this Anti-MaxiK Antibody, aa1184-1200. validated for use in IH, IP & WB.

Key Applications

Immunohistochemistry
Immunoprecipitation
Western Blotting

Application Notes

Western blot: 1:500 using ECL on rat brain membranes.

Immunohistochemistry: On rat brain sections.

Immunoprecipitation: 4 μg using rat brain lysate.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Purified peptide from amino acids 1184-1200 of mouse KCa1.1 .
Epitope	C-terminal intracellular region, STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes MaxiK, amino acids 1184-1200 (K_CA1.1, BK_CA, Large conductance Ca²⁺-activated K⁺ channel, BKCa, Maxi K⁺, KCNMA1).
Species Reactivity	Rat
Species Reactivity Note	The immunogen sequence is identical in human, bovine, chicken, and 16 of 17 amino acids identical in dog.
Antibody Type	Polyclonal Antibody
Entrez Gene Summary	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
Gene Symbol	KCNMA KCNMA1 SLO1 KCa1.1 MAXIK
Purification Method	ImmunoAffinity Purified
UniProt Number	Q08460 Q12791
UniProt Summary	FUNCTION: SwissProt: Q12791 # Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). SIZE: 1236 amino acids; 137560 Da SUBUNIT: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. DOMAIN: SwissProt: Q12791 The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. & The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. & The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. & The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity). & The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel. PTM: Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity. DISEASE: SwissProt: Q12791 # Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders, affecting more than 40 million people worldwide. Paroxysmal dyskinesias are another heterogeneous group of neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. The basic pathophysiology underlying the coexistence of these two disorders is caused by mutations in the KCNMA1 gene. SIMILARITY: Belongs to the potassium channel family. Calcium- activated subfamily. & Contains 1 RCK N-terminal domain. MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).

Biological Information

Immunogen

Purified peptide from amino acids 1184-1200 of mouse KCa1.1 .

Epitope

C-terminal intracellular region, STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200.

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes MaxiK, amino acids 1184-1200 (K_CA1.1, BK_CA, Large conductance Ca²⁺-activated K⁺ channel, BKCa, Maxi K⁺, KCNMA1).

Species Reactivity

Species Reactivity Note

The immunogen sequence is identical in human, bovine, chicken, and 16 of 17 amino acids identical in dog.

Antibody Type

Polyclonal Antibody

Entrez Gene Summary

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Symbol

KCNMA
KCNMA1
SLO1
KCa1.1
MAXIK

Purification Method

ImmunoAffinity Purified

UniProt Number

UniProt Summary

FUNCTION: SwissProt: Q12791 # Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

SIZE: 1236 amino acids; 137560 Da

SUBUNIT: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity.

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

TISSUE SPECIFICITY: Widely expressed. Except in myocytes, it is almost ubiquitously expressed.

DOMAIN: SwissProt: Q12791 The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. & The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. & The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. & The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity). & The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.

PTM: Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.

DISEASE: SwissProt: Q12791 # Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders, affecting more than 40 million people worldwide. Paroxysmal dyskinesias are another heterogeneous group of neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. The basic pathophysiology underlying the coexistence of these two disorders is caused by mutations in the KCNMA1 gene.

SIMILARITY: Belongs to the potassium channel family. Calcium- activated subfamily. & Contains 1 RCK N-terminal domain.

MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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