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Rb X slob2

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货号：AB9778-200UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB9778-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Sloβ2 Antibody
Alternate Names
  • KCNMB2
  • Ca2+-activated K+ channel β subunit 2
  • BKCa β subunit 2
References
Product Information
Format Affinity Purified
Control
  • Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application Anti-Sloβ2 Antibody is an antibody against Sloβ2 for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:200 using ECL on rat kidney and heart membranes.
Immunohistochemistry: On rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified peptide from amino acids 14-32 of human sloβ2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Species Reactivity
  • Rat
Species Reactivity Note The immunogen sequence is identical in dog and 18 of 19 amino acids identical in mouse.
Antibody Type Polyclonal Antibody
Entrez Gene Summary MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which decreases the activation time of MaxiK alpha subunit currents. Two variants encoding the same protein have been found for this gene.
Gene Symbol
  • KCNMB2
  • BKbeta2
  • Hbeta2
  • Hbeta3
  • SLObeta2
  • KCMB2
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9Y691 # Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons.
SIZE: 235 amino acids; 27130 Da
SUBUNIT: Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.
DOMAIN: SwissProt: Q9Y691 The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C- terminus.
PTM: N-glycosylated.
SIMILARITY: Belongs to the KCNMB family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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