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Anti-TWIK-1 (rabbit polyclonal)

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货号：AB9780-200UL

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, R IHC, WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB9780-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-TWIK-1 Antibody
Alternate Names
  • K2p1.1
  • KCNK1
  • Inward rectifying K+ channel TWIK-1
References
Product Information
Format Affinity Purified
Control
  • Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Applications
Application This Anti-TWIK-1 Antibody is validated for use in IH, WB for the detection of TWIK-1.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:200 using ECL on rat brain lysate and 1:300 on HEK-TWIK-1 transfected cells.
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified peptide from amino acids 53-69 of human TWIK-1 (Accession number O00180).
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes TWIK-1 (K2p1.1, KCNK1, Inward rectifying K+ channel TWIK-1).
Species Reactivity
  • Mouse
  • Rat
Species Reactivity Note The immunogen sequence is identical in human and porcine.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity.
Gene Symbol
  • KCNK1
  • K2p1.1
  • HOHO1
  • TWIK-1
  • TWIK1
  • HOHO
  • DPK
  • KCNO1
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: O00180 # Weakly inward rectifying potassium channel.
SIZE: 336 amino acids; 38143 Da
SUBUNIT: Homodimer (Potential).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney.
SIMILARITY: SwissProt: O00180 ## Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
MISCELLANEOUS: Inhibited by barium, quinine, quinidine and internal acidification. Activated by protein kinase C.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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