Anti-Bcl-2

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Ca, Fe	FC, IHC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, Ca, Fe

FC, IHC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-729
Replaces	04-436
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Bcl2 Antibody, clone 100
Alternate Names	B-cell CLL/lymphoma 2 B-cell lymphoma protein 2
Background Information	The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.

Description

Catalogue Number

05-729

Replaces

04-436

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Bcl2 Antibody, clone 100

Alternate Names

B-cell CLL/lymphoma 2
B-cell lymphoma protein 2

Background Information

The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.

Product Information
Format	Purified
Control	Tonsil tissue.
Presentation	Purified mouse monoclonal IgG1 in buffer containing 70% storage buffer PBS with 0.1% sodium azide and 30% glycerol. Store at -20ºC.

Product Information

Format

Purified

Control

Tonsil tissue.

Presentation

Purified mouse monoclonal IgG1 in buffer containing 70% storage buffer PBS with 0.1% sodium azide and 30% glycerol. Store at -20ºC.

Applications
Application	Detect Bcl2 using this Anti-Bcl2 Antibody, clone 100 validated for use in FC, IH & WB.
Key Applications	Flow Cytometry Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry: An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues. FACS Analysis: Recommended

Applications

Application

Detect Bcl2 using this Anti-Bcl2 Antibody, clone 100 validated for use in FC, IH & WB.

Key Applications

Flow Cytometry
Immunohistochemistry
Western Blotting

Application Notes

Immunohistochemistry:
An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues.

FACS Analysis:
Recommended

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.
Epitope	a.a. 41-54
Clone	100
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes Bcl2, Mr 26 kDa.
Isotype	IgG1
Species Reactivity	Human Canine Feline
Species Reactivity Note	Human. Predicted to cross-react with feline and canine, based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_000633
Entrez Gene Summary	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Gene Symbol	BCL2 Bcl-2
Purification Method	Protein G Purified
UniProt Number	P10415
UniProt Summary	FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). SIZE: 239 amino acids; 26266 Da SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1. PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. SIMILARITY: Belongs to the Bcl-2 family.
Molecular Weight	26 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.

Epitope

a.a. 41-54

Clone

100

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes Bcl2, Mr 26 kDa.

Isotype

IgG1

Species Reactivity

Human
Canine
Feline

Species Reactivity Note

Human. Predicted to cross-react with feline and canine, based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_000633

Entrez Gene Summary

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Gene Symbol

BCL2
Bcl-2

Purification Method

Protein G Purified

UniProt Number

P10415

UniProt Summary

FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
SIMILARITY: Belongs to the Bcl-2 family.

Molecular Weight

26 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on Raji RIPA cell lysate. Western Blot Analysis: 0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by western blot on Raji RIPA cell lysate.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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