Anti-CENP-E, clone177 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, IHC, WB, IP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

ICC, IHC, WB, IP

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1502
Description	Anti-CENP-E Antibody, clone177
Alternate Names	centromere protein E, 312kDa kinesin family member 10 centromere protein E (312kD) Kinesin-related protein CENPE Centromere autoantigen E (312kD) Centromeric protein E centromere protein E
Background Information	Centromeric protein E (CENP-E) is a mitosis-specific kinesin belonging to the kinesin-7 family. CENP-E, along with cytoplasmic dyenin, is one of two main microtubule motors known to localize at kinetochores. During mitosis, CENP-E has an active role in the migration of chromosomes toward the metaphase plate, and is directly responsible for microtubule capture and stabilization by kinetochores. Reduction in CENP-E expression results in the high occurrence of whole chromosome missegregation, and its removal or inhibition leads to the presence of unattached chromosomes near the spindle pole as well as a significant reduction in the number of microtubules captured by kinetochores.

Description

Catalogue Number

05-1502

Description

Anti-CENP-E Antibody, clone177

Alternate Names

centromere protein E, 312kDa
kinesin family member 10
centromere protein E (312kD)
Kinesin-related protein CENPE
Centromere autoantigen E (312kD)
Centromeric protein E
centromere protein E

Background Information

Centromeric protein E (CENP-E) is a mitosis-specific kinesin belonging to the kinesin-7 family. CENP-E, along with cytoplasmic dyenin, is one of two main microtubule motors known to localize at kinetochores. During mitosis, CENP-E has an active role in the migration of chromosomes toward the metaphase plate, and is directly responsible for microtubule capture and stabilization by kinetochores. Reduction in CENP-E expression results in the high occurrence of whole chromosome missegregation, and its removal or inhibition leads to the presence of unattached chromosomes near the spindle pole as well as a significant reduction in the number of microtubules captured by kinetochores.

Product Information
Format	Purified
Control	HeLa cells
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Purified

Control

HeLa cells

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Use Anti-CENP-E Antibody, clone177 (Mouse Monoclonal Antibody) validated in ICC, IHC, WB, IP to detect CENP-E also known as centromere protein E (312kD).
Key Applications	Immunocytochemistry Immunohistochemistry Western Blotting Immunoprecipitation
Application Notes	Immunohistochemistry Analysis: 1:100 dilution from a representative lot of this antibody detected CENP-E in kidney and malignant melanoma tissue. Western Blot Analysis: A representative lot of this antibody was used by an independent laboratory in WB. (Yen, T.J., et al. (1991). EMBO J. 10(5):1245–1254.)

Applications

Application

Use Anti-CENP-E Antibody, clone177 (Mouse Monoclonal Antibody) validated in ICC, IHC, WB, IP to detect CENP-E also known as centromere protein E (312kD).

Key Applications

Immunocytochemistry
Immunohistochemistry
Western Blotting
Immunoprecipitation

Application Notes

Immunohistochemistry Analysis:
1:100 dilution from a representative lot of this antibody detected CENP-E in kidney and malignant melanoma tissue.

Western Blot Analysis: A representative lot of this antibody was used by an independent laboratory in WB. (Yen, T.J., et al. (1991). EMBO J. 10(5):1245–1254.)

Biological Information
Immunogen	Native protein corresponding to human CENP-E.
Epitope	Unknown
Clone	177
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes CENP-E.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001804
Entrez Gene Summary	Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq].
Gene Symbol	CENP-E3 CENP-E
Purification Method	Protein G Purified
UniProt Number	Q02224
UniProt Summary	FUNCTION: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. SUBUNIT STRUCTURE: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1. SUBCELLULAR LOCATION: Kinetochore. Cytoplasm › cytoskeleton › spindle. Centromere. Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. DOMAIN: The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part (By similarity). PTM: The C-teminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity). Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore. SEQUENCE SIMILARITIES: Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain. Contains 25 LRR (leucine-rich) repeats.
Molecular Weight	312 kDa Calculated

Biological Information

Immunogen

Native protein corresponding to human CENP-E.

Epitope

Unknown

Clone

177

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes CENP-E.

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001804

Entrez Gene Summary

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq].

Gene Symbol

CENP-E3
CENP-E

Purification Method

Protein G Purified

UniProt Number

Q02224

UniProt Summary

FUNCTION: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.

SUBUNIT STRUCTURE: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1.

SUBCELLULAR LOCATION: Kinetochore. Cytoplasm › cytoskeleton › spindle. Centromere. Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

DOMAIN: The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part (By similarity).

PTM: The C-teminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity).

Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.

SEQUENCE SIMILARITIES: Belongs to the kinesin-like protein family.

Contains 1 kinesin-motor domain.

Contains 25 LRR (leucine-rich) repeats.

Molecular Weight

312 kDa Calculated

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: 1:500 dilution of this antibody detected CENP-E in HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected CENP-E in HeLa cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CENP-E, clone177 (mouse monoclonal)

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