Osteogenesis Quantitation Kit

Detection Methods
Chromogenic

Detection Methods

Chromogenic

Description
Catalogue Number	ECM815
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Osteogenesis Quantitation Kit
Overview	CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit. The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates. For Research Use Only. Not for use in diagnostic procedures.
Materials Required but Not Delivered	• Phosphate-Buffered Saline (1X PBS) • Fixative for Alizarin Red Staining (e.g. 10% formaldehyde, or 4% paraformaldehyde, or 70% ethanol) • Hemocytometer • Microscope • Plate reader capable of reading at 405 nm

Description

Catalogue Number

ECM815

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Osteogenesis Quantitation Kit

Overview

CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.

The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.

For Research Use Only. Not for use in diagnostic procedures.

Materials Required but Not Delivered

• Phosphate-Buffered Saline (1X PBS)
• Fixative for Alizarin Red Staining
(e.g. 10% formaldehyde, or 4% paraformaldehyde, or 70% ethanol)
• Hemocytometer
• Microscope
• Plate reader capable of reading at 405 nm

Product Information
Components	Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C. 10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C. 10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C. 10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. Store at 2-8°C.
Detection method	Chromogenic

Product Information

Components

Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C.
10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. Store at 2-8°C.

Detection method

Chromogenic

Applications
Application	The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.
Application Notes	Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810 One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.

Applications

Application

The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.

Application Notes

Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	All components should be stored at 2 - 8ºC until expiration date printed on label.

Storage and Shipping Information

Storage Conditions

All components should be stored at 2 - 8ºC until expiration date printed on label.

Packaging Information
Material Size	1 kit

Packaging Information

Material Size

1 kit

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Osteogenesis Quantitation Kit

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