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Anti-HSP27 Phospho (Ser78)

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货号：04-447

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-645
Replaces 04-447
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-HSP27 (Ser78) Antibody, clone JBW502
References
Product Information
Format Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Anti-phospho-HSP27 (Ser78) Antibody, clone JBW502 is an antibody against phospho-HSP27 (Ser78) for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen peptide corresponding to amino acids 73-83 of human HSP27 with a C-terminus cysteine added for conjugation (YSRAL[pS]RQLSS-C)
Clone JBW502
Host Mouse
Specificity phosphorylated HSP27 (Ser78)
Isotype IgG
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSPB1
  • Hs.76067
  • HSP27
  • Hsp25
  • HspB1
  • HSP28
  • CMT2F
  • HS.76067
  • DKFZp586P1322
  • HMN2B
  • SRP27
Modifications
  • Phosphorylation
Purification Method Protein G Chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
Molecular Weight 27kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on phosphorylated HSP27 in 20mg of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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