Ebp1 (N-terminus) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ICC	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ICC

Serum

Polyclonal Antibody

Description
Catalogue Number	ABE43
Replaces	07-397
Description	Anti-Ebp1 NT Antibody
Alternate Names	Proliferation-associated protein 2G4 Cell cycle protein p38-2G4 homolog hG4-1 ErbB3-binding protein 1 Cell cycle protein p38-2G4 homolog ErbB-3 binding protein 1 ErbB3-binding protein Ebp1
Background Information	Ebp1 is an RNA-binding protein involved in growth regulation. In cancer cells, this protein and has been implicated in growth inhibition and differentiation. Ebp1 is known to bind ribosomal and double-stranded RNA. It is a found in pre-ribosomal ribonucleoprotein complexes and is believed to play a role in ribosome assembly and rRNA processing. This protein is also a component of cytoplasmic bcl-2 messenger ribonucleoprotein. Through interactions with histion deacetylases, this protein can act transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes. Ebp1 also interacts with the cytoplasmic domain of the ErbB3 receptor, potentially transducing regulatory growth signals.

Description

Catalogue Number

ABE43

Replaces

07-397

Description

Anti-Ebp1 NT Antibody

Alternate Names

Proliferation-associated protein 2G4
Cell cycle protein p38-2G4 homolog
hG4-1
ErbB3-binding protein 1
Cell cycle protein p38-2G4 homolog
ErbB-3 binding protein 1
ErbB3-binding protein Ebp1

Background Information

Ebp1 is an RNA-binding protein involved in growth regulation. In cancer cells, this protein and has been implicated in growth inhibition and differentiation. Ebp1 is known to bind ribosomal and double-stranded RNA. It is a found in pre-ribosomal ribonucleoprotein complexes and is believed to play a role in ribosome assembly and rRNA processing. This protein is also a component of cytoplasmic bcl-2 messenger ribonucleoprotein. Through interactions with histion deacetylases, this protein can act transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes. Ebp1 also interacts with the cytoplasmic domain of the ErbB3 receptor, potentially transducing regulatory growth signals.

Product Information
Format	Serum
Control	A431 Cell Lysate.
Presentation	Rabbit Polyclonal Serum with 0.05% sodium azide

Product Information

Format

Serum

Control

A431 Cell Lysate.

Presentation

Rabbit Polyclonal Serum with 0.05% sodium azide

Applications
Application	Anti-Ebp1 N-terminal Antibody is a Rabbit Polyclonal Antibody for detection of Ebp1 also known as Proliferation-associated protein 2G4 & has been validated in WB, ICC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry: A 1:500 dilution from a representative lot detected Ebp1 in NIH/3T3, A431, and HeLa cells.

Applications

Application

Anti-Ebp1 N-terminal Antibody is a Rabbit Polyclonal Antibody for detection of Ebp1 also known as Proliferation-associated protein 2G4 & has been validated in WB, ICC.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry:
A 1:500 dilution from a representative lot detected Ebp1 in NIH/3T3, A431, and HeLa cells.

Biological Information
Immunogen	Linear Peptide corresponding to the N-terminus of Ebp1.
Epitope	N-terminus
Host	Rabbit
Specificity	Recognizes Ebp-1.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006182
Entrez Gene Summary	This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]
Gene Symbol	EBP1 HG4-1 p38-2G4 PA2G4
Purification Method	Unpurified
UniProt Number	Q9UQ80
UniProt Summary	Function: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) By similarity. Ref.8 Ref.10 Ref.11 Ref.12 Subunit structure: Interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Ref.8 Ref.10 Ref.12 Ref.2 Ref.7 Ref.19 Subcellular location: Cytoplasm. Nucleus › nucleolus. Note: Tranlocates to the nucleus upon treatment with HRG. Ref.11 Ref.2 Ref.9 Tissue specificity: Expressed in several cell lines tested, including primary and transformed cell lines. Ref.11 Post-translational modification: Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Ref.7 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Miscellaneous: Does not contain metal cofactors and does not have aminopeptidase activity. Sequence similarities: Belongs to the peptidase M24 family.
Molecular Weight	48 kDa Observed

Biological Information

Immunogen

Linear Peptide corresponding to the N-terminus of Ebp1.

Epitope

N-terminus

Host

Rabbit

Specificity

Recognizes Ebp-1.

Isotype

IgG

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006182

Entrez Gene Summary

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]

Gene Symbol

EBP1
HG4-1
p38-2G4
PA2G4

Purification Method

Unpurified

UniProt Number

Q9UQ80

UniProt Summary

Function: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) By similarity. Ref.8 Ref.10 Ref.11 Ref.12

Subunit structure: Interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Ref.8 Ref.10 Ref.12 Ref.2 Ref.7 Ref.19

Subcellular location: Cytoplasm. Nucleus › nucleolus. Note: Tranlocates to the nucleus upon treatment with HRG. Ref.11 Ref.2 Ref.9

Tissue specificity: Expressed in several cell lines tested, including primary and transformed cell lines. Ref.11

Post-translational modification: Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Ref.7 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18

Miscellaneous: Does not contain metal cofactors and does not have aminopeptidase activity.

Sequence similarities: Belongs to the peptidase M24 family.

Molecular Weight

48 kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in A431 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in A431 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Ebp1 (N-terminus) (rabbit polyclonal)

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