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CAFFEINE 10G

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货号：19-156

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP, ICC, IHC M Purified Monoclonal Antibody
Description
Catalogue Number MABE152
Description Anti-SRp55 Antibody, clone 9-1-56
Alternate Names
  • splicing factor, arginine/serine-rich 6
  • arginine/serine-rich splicing factor 6
  • splicing factor, arginine/serine-rich, 55 kDa
  • Pre-mRNA-splicing factor SRP55
  • pre-mRNA splicing factor SRP55
Background Information Pre-mRNA-splicing factor SRp55 is a member of the serine/arginine-rich (SR) protein family. This group of proteins is found mainly in the nucleus where they play a critical role in regulated and constitutive splicing of precursor mRNAs. SRp55 is involved in the constitutive process for mRNA splicing, and is able to mediate alternative splice site selection. Considered one of the more ubiquitous of the splice factors, SRp55 is upregulated in response to DNA damage when p53 is lacking. SRp55 is significantly phosphorylated on RS domain serine residues.
References
Product Information
Format Purified
Control
  • HeLa cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Applications
Application Use Anti-SRp55 Antibody, clone 9-1-56 (Mouse Monoclonal Antibody) validated in WB, IP, ICC, IHC to detect SRp55 also known as arginine/serine-rich splicing factor 6, Pre-mRNA-splicing factor SRP55.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Biological Information
Immunogen MBP-tagged recombinant protein corresponding to human SRp55.
Epitope Not determined
Clone 9-1-56
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants.
Gene Symbol
  • SRP55
  • SFRS6
  • B52
Purification Method Protein G
UniProt Number
UniProt Summary FUNCTION: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.

SUBUNIT STRUCTURE: Binds SFRS12.

SUBCELLULAR LOCATION: Nucleus.

PTM: Extensively phosphorylated on serine residues in the RS domain (By similarity).

SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight ~ 43 kDa observed. 40 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SRp55 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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