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Anti-IL-21 (rabbit polyclonal)

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货号：ABF184

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, FC Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABF184
Description Anti-IL-21 Antibody
Alternate Names
  • Interleukin-21
  • IL-21
  • Za11
Background Information Interleukin-21 (UniProt Q9HBE4; also known as IL-21, Za11) is encoded by the IL21 (also known as CVID11) gene (Gene ID 59067) in human. IL-21 is a type I four-alpha-helical bundle cytokine primarily produced by CD4+ T cells and NKT cells. The heterodimeric receptor IL-21R is dominantly expressed by B cells and shares the common cytokine receptor gamma chain (γc) with receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Studies indicate that IL-21 potently regulates both adaptive and innate immune responses and possesses widely pleiotropic functions. IL-21 has also been linked to autoimmune disorders, allergies, inflammatory diseases, as well as the pathogenesis of cancer. Human IL-21 is initially translated with an N-terminal 22 aa signal peptide sequence, which is later removed to produce the 133 aa mature IL-21.
References
Product Information
Format Affinity Purified
Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This rabbit Polyclonal Anti-IL-21 Antibody, Cat. No. ABF184 is validated for use in Western Blotting, Flow Cytometry, for the detection of IL-21.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application Notes Flow Cytometry Analysis: 3.0 µg of this antibody detected IL-21 in human peripheral blood mononuclear cells.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to human IL-21 sequence near the C-terminus.
Epitope Near C-terminus
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity Expected to react with pro- and mature IL-21 of both isoform 1 and isoform 2 (IL-21iso).
Species Reactivity
  • Human
Species Reactivity Note Human. Predicted to react with Bovine, Porcine based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL21
Purification Method Affinity Purfied
UniProt Number
Molecular Weight ~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in human IL-21 transfected HEK293 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-21 in 10 µg of human IL-21 transfected HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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