Anti-Calpain I/II, large subunit, clone 1D10A7 - 炼石商城

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Anti-Calpain I/II, large subunit, clone 1D10A7

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货号：MABS1215

品牌：Millipore 密理博

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, Rb, Ch, Mk, H	WB, ICC	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MABS1215
Description	Anti-Calpain I/II, large subunit Antibody, clone 1D10A7
Alternate Names	Calpain-1 catalytic subunit Calcium-activated neutral proteinase 1 CANP 1 Calpain mu-type Calpain-1 large subunit Cell proliferation-inducing gene 30 protein Micromolar-calpain muCANP Calpain I/II large subunit
Background Information	Calpains I & II are intracellular cysteine proteinases distributed in various tissues and cells. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca+2 concentrations and calpain II at millimolar Ca+2 concentrations. Both calpains are composed of a large catalytic (80 kDa) and a small regulatory (30 kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain activity was initially characterized as “calcium-activated neutral protease” (CANP) detected in brain, lens of the eye, and other tissues. The name “calpain” was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.

References

Product Information
Format	Ascites
Presentation	Mouse monoclonal IgG2aκ ascites with 0.05% sodium azide.

Applications
Application	Detect CAPN1 using this mouse monoclonal Anti-Calpain I/II, large subunit Antibody, clone 1D10A7, Cat. No. MABS1215, validated for use in Western Blotting and Immunocytochemistry.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Western Blotting Analysis: A 1:20,000 dilution from a representative lot detected recombinant human calpain-2 and calpain-1 C115S mutant protein. Western Blotting Analysis: A 1:20,000 dilution from a representative lot detected Calpain, large subunit in rat spleen tissue lysate. Immunocytochemistry Analysis: A representative lot detected Calpain, large subunit in mouse embryonic fibroblast (MEF) cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207). Western Blotting Analysis: A representative lot detected Calpain, large subunit in cytosolic preparation from rat cortical tissue (Blomgren, K., et al. (1995). Brain Res. 684(2):143-149). Western Blotting Analysis: A representative lot detected purfied mu- and m-calpain large subunits from rabbit, monkey, human, and rat tissues (Kawashima, S., et al. (1998). Biol Chem. 379(2):201-204). Western Blotting Analysis: A representative lot detected Calpain, large subunit in normal & cataractous rat lens homogenates (Inomata, M., et al. (2009). Nat Med. 15(10):1202-1207). Western Blotting Analysis: A representative lot detected Calpain, large subunit in MEF cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207).

Biological Information
Immunogen	Purified m-calpain from rabbit skeletal muscle.
Clone	1D10A7
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 1D10A7 detects the large catalytic (80K), but not the small regulatory (30K), subunit of mu-calpain (calpain I) and m-calpain (calpain II), with ~7-fold higher affinity toward m-calpain than mu-calpain.
Isotype	IgG2aκ
Species Reactivity	Rat Rabbit Chicken Monkey Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001185797
Gene Symbol	CAPN1 CANPL1 PIG30
Purification Method	Unpurified
UniProt Number	P07384
Molecular Weight	~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in rat lung tissue lysate. Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Calpain, large subunit in rat lung tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	50 µL

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Supplemental Information

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