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CDK1/CyclinB1 (Active Enzyme)

市场价：: 0.00

促销价：: ¥0.00

货号：14-450-D

品牌：Millipore 密理博

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北京朝阳

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Replacement Information

重要规格表

Key Applications	Entrez Gene Number	Species	Uni Prot Number
KA	CDK1: NM_001786, CyclinB1: NM_031966	Human	P06493

Description
Catalogue Number	14-450-D
Brand Family	Upstate
Trade Name	Upstate
Description	CDK1/CyclinB1
Overview	Recombinant human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively, full-length with an N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system

References

Product Information
Presentation	50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

Applications
Application	Recombinant human CDK1/CyclinB1 complex, also known as CDC and CCNB1, CCNB respectively, full-length with an N-terminal GST-tags on both proteins, expressed in a baculovirus-infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications	Kinase Assay

Biological Information
Concentration	0.1 mg/ml
Purity	≥75%
Source	Sf9 Insect Cells
Specific Activity	90 pmol/min/μg
Entrez Gene Number	CDK1: NM_001786 CyclinB1: NM_031966
Entrez Gene Summary	The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Gene Symbol	CDC2 CDK1 MGC111195 DKFZp686L20222 CDC28A
Target Sub-Family	CMGC
UniProt Number	P06493
UniProt Summary	FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. SIZE: 297 amino acids; 34095 Da SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. SUBCELLULAR LOCATION: Nucleus (By similarity). SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight	CDK1: 58 kDa, CyclinB1: 75 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Kinase assay: 10 µl of CDK1/CyclinB1 was mixed with 5 µl Histone H1 substrate (1 mg/ml) and 5 µl water. Reaction was initiated by adding 5 µl of [33P]-ATP cocktail and incubated at 30°C for 15 min. Reaction was read by adding 20 µl to P81 paper and read in the presence of scintillation fluid in a scintillation counter.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for > 6 month at –80°C from date of receipt.

Packaging Information
Material Size	10 μg
Material Package	Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M-D when ordering the 250 µg size.

Transport Information

Supplemental Information

Specifications

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