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CDK5/p35 (Active Enzyme)

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货号：14-477-D

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-477
Brand Family Upstate
Trade Name
  • Upstate
Description Cdk5/p35 Protein, active, 10 µg
Overview Complex of recombinant fulllength human cdk5 containing and N-terminal His6-tag and recombinant full-length human p35 containing an N-terminal GST-tag
References
Product Information
Applications
Application Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Source Both are expressed by baculovirus in Sf21 insect cells
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • CDK5
  • PSSALRE
Protein Target CDK5/p35
Purification Method Ni-NTA agarose
Target Sub-Family CMGC
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q00535 # Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
SIZE: 292 amino acids; 33304 Da
SUBUNIT: Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, growth cone (By similarity). Note=In axonal growth cone with extension to the peripheral lamellipodia (By similarity).
SIMILARITY: SwissProt: Q00535 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight 34kDa (cdk5), 61kDa (p35 with GST-tag)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -70°C
Packaging Information
Material Size 10 µg
Material Package Also available in 250μg size as catalog # 14-477M - Call for pricing and availability.
Transport Information
Supplemental Information
Specifications

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