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cRAF (RAF1) (Active Enzyme)

市场价：: 0.00

促销价：: ¥0.00

货号：14-352-D

品牌：Millipore 密理博

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Replacement Information

Description
Catalogue Number	14-352-D
Brand Family	Upstate
Trade Name	Upstate
Description	cRAF (RAF1)
Overview	Recombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag

References

Product Information
Presentation	40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT

Applications
Application	Recombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications	Kinase Assay

Biological Information
Concentration	0.71 mg/ml
Purity	≥70%
Source	Sf9 insect cells
Specific Activity	158 pmol/min/µg
Entrez Gene Number	NM_002880
Entrez Gene Summary	Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
Gene Symbol	RAF1 C-RAF cRaf Raf-1 c-Raf CRAF RAF
Target Sub-Family	TKL
UniProt Number	P04049
UniProt Summary	FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. COFACTOR: Binds 2 zinc ions per subunit. SIZE: 648 amino acids; 73052 Da SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight	65 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Kinase assay: 10 μM ATP, 1 μM MEK1(K97R), 1 hour.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for > 6 month at –80°C from date of receipt.

Packaging Information
Material Size	10 μg
Material Package	Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.

Transport Information

Supplemental Information

Specifications

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