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SRC (Active Enzyme)

市场价：: 0.00

促销价：: ¥0.00

货号：14-326-D

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

Description
Catalogue Number	14-326-D
Brand Family	Upstate
Trade Name	Upstate
Description	SRC
Overview	Recombinant human SRC, full length with N-terminal HIS tag

References

Product Information
Presentation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 106 mM imidazole and 20% glycerol

Applications
Application	Recombinant human SRC, full length with N-terminal HIS tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications	Kinase Assay

Biological Information
Concentration	1.2 mg/ml
Purity	≥80%
Source	Sf9 insect cells
Specific Activity	205 pmol/min/µg
Entrez Gene Number	NM_005417
Entrez Gene Summary	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol	SRC ASV c-src p60-Src SRC1 c-SRC pp60c-src
Target Sub-Family	TK
UniProt Number	P12931
UniProt Summary	SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight	61 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Kinase assay: Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 mM ATP. 35 min at room temperature.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for > 6 month at –80°C from date of receipt.

Packaging Information
Material Size	10 μg
Material Package	2 vials at 5μg each Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.

Transport Information

Supplemental Information

Specifications

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