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AMPK (伪1,尾1,纬1) (Active Enzyme)

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货号：14-840-D

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-840-D
Brand Family Upstate
Trade Name
  • Upstate
Description AMPK (A1/B1/G1)
Overview Recombinant full-length human AMPK (combination of A1/B1/G1 subunits), with C-terminal His tag.
References
Product Information
Presentation 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Applications
Application Recombinant full-length human AMPK (combination of A1/B1/G1 subunits), with C-terminal His tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration 0.1 mg/ml
Purity ≥50%
Source Sf9 insect cells
Specific Activity 816 nmol/min/mg
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol
  • PRKAB1
  • PRKAG1
  • PRKAA1
Target Sub-Family CAMK
UniProt Number
Molecular Weight 68kDa (A1), 38kDa (B1), and 40kDa (G1)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Kinase assay: Buffer: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate AMPK A1/B1G1 with 0.2 mg/ml SAMStide peptide substrate, 0.1 mM AMP, and [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size 10 μg
Transport Information
Supplemental Information
Specifications

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