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Anti-Integrin alpha6, clone 6H7.1 (mouse monoclonal)

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货号：MABT356

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number MABT356
Description Anti-Integrin alpha6 Antibody, clone 6H7.1
Alternate Names
  • Integrin alpha-6
  • CD49 antigen-like family member F
  • VLA-6
  • CD49f
Background Information Integrin alpha-6 (UniProt P23229; also known as CD49 antigen-like family member F, CD49f, Integrin alpha6B, VLA-6) is encoded by the ITGA6 (also known as ITGA6B) gene (Gene ID 3655) in human. The biological functions attributed to cell-extracellular matrix (ECM) interactions are mediated primarily by integrin family transmembrane receptors. A total of 18 alpha and 8 beta integrins have been identified in human. Each alpha integrin pairs up with one beta integrin non-covalently to form a functional heterodimeric dimer. At least 24 distinct integrin heterodimers are known with varying ligand specificities. For example, the alpha6-beta1 integrin is reported to mediate the spreading and laminin-binding of Schwann cells, while the alpha6-beta4 integrin is shown to play a role in the suppression of anoikis in human intestinal epithelial cells (IECs).

Signal (1-23), Integrin alpha-6 (24-1130). Processed into Integrin alpha-6 heavy chain (24 – 938), Integrin alpha-6 light chain (942-1130), Processed integrin alpha-6 (Alpha6p; 636 – 1130)
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Integrin alpha6 Antibody, clone 6H7.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Integrin alpha6.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Integrin alpha6 in human skin tissue.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the cytoplasmic region of human Integrin alpha6 isoforms 2/4/6/7/9.
Epitope Cytoplasmic region of isoforms 2/4/6/7/9
Clone 6H7.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Expected to react with Integrin alpha-6 spliced isoforms 2 (Alpha-6X1A), 4 (Alpha-6X2A ), 6 (Alpha-6X1X2A), 7, and 9, as well as their respective proforms, cleaved light chains, and processed integrin alpha-6 forms (Alpha6p). Epitope is not present in isoform 1 (Alpha-6X1X2B), 3 (Alpha-6X1B), or 5 (Alpha-6X2B).
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGA6
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~140 kDa observed. Due to glycosylation, the target band(s) appears larger than the calculated 106.6 to 124.0 kDa based on the proform sequences of isoforms 2/4/6/7/9. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in SW480 human colon cancer cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin alpha6 in 10 µg of SW480 human colon cancer cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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