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Anti-VAPA, clone 7E10.1 (mouse monoclonal)

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货号：MABN361

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IH(P) M Purified Monoclonal Antibody
Description
Catalogue Number MABN361
Description Anti-VAPA Antibody, clone 7E10.1
Alternate Names
  • Vesicle-associated membrane protein-associated protein A
  • VAMP-A
  • VAMP-associated protein A
  • VAP-A
  • 33 kDa VAMP-associated protein
  • VAP-33
  • VAPA
Background Information Vesicle-associated membrane protein-associated protein A (UniProt Q9P0L0; also known as 33 kDa VAMP-associated protein, hVAP-33, VAMP-A, VAMP-associated protein A, VAP-33, VAP-A) is encoded by the VAPA (also known as VAP33) gene (Gene ID 9218) in human. VAMP-associated proteins (VAPs) are type IV membrane proteins that are well conserved among species. There exist three human VAPs encoded by two genes, with VAPA encoding VAP-A and VAPB encoding VAP-B and VAP-C. VAPs generally localize at the endoplasmic reticulum (ER), although they are also reported to localize at other subcellular organelles in some species and cell types. VAP-A contains a major sperm protein (MSP) domain at the N-terminal, followed by a coiled-coil domain, and a transmembrane (TM) domain. VAPs were shown to have important roles in non-vesicular lipid transport, lipid metabolism, the regulation of ER structure, and the unfolded protein response through MSP domain-mediated interaction with FFAT motifs. Oxysterol-binding protein (OSBP) is a cytosolic receptor of cholesterol and oxysterols. OSBP is recruited to the ER by binding to the MSP domain of VAP-A, a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-VAPA Antibody, clone 7E10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of VAPA.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application Notes Western Blotting Analysis: 0.2 µg/mL from a representative lot detected VAPA in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected VAPA in human cerebellum and human testis tissue.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to the C-terminal half of human VAPA.
Epitope C-terminal half
Clone 7E10.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Cross-reactivity to human VAPA spliced isoform 2 is highly expected, but not confirmed.
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VAPA
  • VAP33
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~30 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected VAPA in 10 µg of SH-SY5Y cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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