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Anti-Bmi-1, clone F6, Alexa Fluor?647 Conjugate

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货号：05-637-AF647

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, Mk ICC M AlexaFluor®647 Monoclonal Antibody
Description
Catalogue Number 05-637-AF647
Description Anti-Bmi-1 Antibody, clone F6, Alexa Fluor® 647
Alternate Names
  • Polycomb complex protein BMI-1
  • Polycomb group RING finger protein 4
  • Bmi-1
Background Information Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.
References
Product Information
Format AlexaFluor®647
Presentation Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Applications
Application This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor® 647 is validated for use in Immunocytochemistry for the detection of Bmi-1.
Key Applications
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HepG2 cells.
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
Biological Information
Immunogen Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
Epitope a.a. 1-202
Clone F6
Concentration Please refer to lot specific datasheet.
Host Mouse
Isotype IgG1
Species Reactivity
  • Human
  • Monkey
Species Reactivity Note Human, Monkey. Predicted to react with Mouse, Rat, Rabbit based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Bmi1
  • Bmi-1
  • Pcgf4
Purification Method Protein G Purified
UniProt Number
Molecular Weight 40-44 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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