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Anti-COUP-TF2 (rabbit polyclonal)

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货号：ABE1426

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, H IH(P), IF, ChIP, WB Rb Serum Polyclonal Antibody
Description
Catalogue Number ABE1426
Description Anti-COUP-TF2 Antibody
Alternate Names
  • COUP transcription factor 2
  • COUP-TF2
  • Apolipoprotein AI regulatory protein 1
  • ARP-1
  • COUP transcription factor II
  • COUP-TF II
  • Nuclear receptor subfamily 2 group F member 2
  • COUP-TF2
Background Information COUP transcription factor 2 (UniProt P43135; also known as Apolipoprotein AI regulatory protein 1, Apolipoprotein regulatory protein 1, ARP-1, COUP-TF II, COUP transcription factor II, Nuclear receptor subfamily 2 group F member 2) is encoded by the Nr2f2 (also known as Aporp1, Arp-1, Arp1, Tfcoup2) gene (Gene ID 11819) in murine species. COUP transcription factors1&2 (COUP-TFI & COUP-TFII) are two related nuclear orphan receptors implicated in their roles in guiding migrating neurons towards their target regions during brain development. COUP-TFI and COUP-TFII are expressed in distinct and non-overlapping migrating cells in specific pathways in the basal telencephalon of mouse embryos at a stage when interneuron migration is robust. While COUP-TFI appears to control dorsal and ventral migration in the basal telencephalon, COUP-TFII is more specific in modulating tangential migration into the intermediate zone of the cortex. Coup-TFII is also identified as an activator of Prox1 expression in venous lymphatic endothelial cell (LEC) progenitors during early stages of LEC specification and differentiation.
References
Product Information
Format Serum
Presentation Rabbit polyclonal serum with 0.05% sodium azide.
Applications
Application This Anti-COUP-TF2 Antibody is validated for use in Immunohistochemistry (Paraffin), Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Western Blotting for the detection of COUP-TF2.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
Application Notes Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected COUP-TF2 in mouse cerebral cortex.
Immunofluorescence Analysis: A 1:500-1000 dilution of a representative lot detected COUP-TF2 immunoreactivity in the developing brain using paraformaldehyde-fixed cryosections from wild-type mouse E13.5 embryos, but not from embryos with conditional brain region COUP-TF2-knockout, by fluorescent immunohistochemistry (Courtesy of Dr. Michele Studer, Institut de Biologie Valrose, France).
Immunohistochemistry Analysis: A representative lot detected a different COUP-TF2 expression pattern than that of COUP-TF1 in the developing telencephalon of forebrain using cryosections from E13.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunocytochemistry Analysis: A representative lot detected COUP-TF2I immunoreactivity in reelin-positive Cajal-Retzius cells, but not in calbindin-positive GABAergic interneurons in the developing cortex by dual fluorescent immunohistochemistry using cryosections from E13.5 and E15.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Chromatin Immunoprecipitation Analysis: A representative lot detected COUP-TF2 occupancy at the conserved COUP-TFII binding motif in the regulatory region of Prox1 using chromatin preparations from human lymphatic endothelial cells (LECs) (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Western Blotting Analysis: A representative lot detected COUP-TF2 expression in HEK293T and NIH3T3 cells (Srinivasan, R.S., et al. (2010). Genes Dev. 24(7):696-707).
Biological Information
Immunogen GST-tagged recombinant protein corresponding to the the N-terminal half of mouse COUP-TF2.
Epitope N-terminal half
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity Reacts with COUP-TF2, but not COUP-TF1.
Species Reactivity
  • Mouse
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nr2f2
  • Aporp1
  • Arp-1
  • Arp1
  • Tfcoup2
Purification Method Unpurified
UniProt Number
Molecular Weight 45 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Immunohistochemistry in mouse hippocampus tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected COUP-TF2 in mouse hippocampus tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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