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Anti-CRTC1/TORC-1, clone 5F3.1 (mouse monoclonal)

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号:MABE461

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M, H, R WB, IHC, IP M Purified Monoclonal Antibody
Description
Catalogue Number MABE461
Description Anti-CRTC1 Antibody/TORC-1, clone 5F3.1
Alternate Names
  • CREB-regulated transcription coactivator 1
  • Mucoepidermoid carcinoma translocated protein 1
  • Transducer of regulated cAMP response element-binding protein 1
  • TORC-1
  • Transducer of CREB protein 1
  • CRTC1/TORC-1
Background Information CREB-regulated transcription coactivator 1 (UniProt Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1, TORC-1, Transducer of CREB protein 1, Transducer of regulated cAMP response element-binding protein 1) is encoded by the CRTC1 (also known as KIAA0616, MECT1, TORC1, WAMTP1) gene (Gene ID 23373) in human. CRTC1 belongs to a family of glycosylated Ser/Thr-rich phosphoproteins that bind the basic leucine zipper (bZIP) region of Ca2+/cAMP-responsive element-binding protein (CREB) and function as CREB coactivators. CRTCs participate in CREB-mediated transcriptions of both canonical cellular cAMP response elements (CREs) and the non-canonical viral CREs. CRTC1, specifically, is involved in CREB-mediated transcription of various neuronal immediate early genes (IEGs) as well as CREB-dependent hepatitis B virus (HBV) replication.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 is validated for use in Western Blotting, Immunohistochemistry, Immunoprecipitation for the detection of CRTC1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CRTC1/TORC-1 in 10 µg of mouse hypothalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CRTC1/TORC-1 in human cerebral cortex, rat cerebral cortex, human cerebellum, and rat cerebellum tissue.
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous CRTC1/TORC-1 from mouse hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1, but not CRTC2/TORC-2, from lysates of transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).
Western Blotting Analysis: A representative lot detected endogenous CRTC1/TORC-1 in human, mouse, and rat hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1 in lysates from transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).
Biological Information
Immunogen GST-tagged recombinant protein corresponding to human CRTC1/TORC-1 sequence within the N-terminal half..
Epitope Internal (N-terminal half)
Clone 5F3.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Expected to react with all three human spliced isoforms
Isotype IgG2aκ
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CRTC1
  • KIAA0616
  • MECT1
  • TORC1
  • WAMTP1
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~68 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CRTC1/TORC-1 in 10 µg of mouse cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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