Anti-IFIT3 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	WB, IP, IH(P), FC, ICC	Rb	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, H

WB, IP, IH(P), FC, ICC

Serum

Polyclonal Antibody

Description
Catalogue Number	ABF1048
Description	Anti-IFIT3 Antibody
Alternate Names	Interferon-induced protein with tetratricopeptide repeats 3 IFIT-3 Glucocorticoid-attenuated response gene 49 protein GARG-49 P49 IRG2 IFIT3
Background Information	Interferon-induced protein with tetratricopeptide repeats 3 (UniProt Q64345; also known as GARG-49, Glucocorticoid-attenuated response gene 49 protein. IFIT-3, IRG2, p49) is encoded by the Ifit3 (also known as Garg49, Ifi49, Isg49) gene (Gene ID 5959) in murine species. IFIT1, IFIT2, and IFIT3 constitute the family of interferon-stimulated genes (ISGs) that are induced in response to virus infection, stimulation by interferons (IFNs), or double-stranded RNA (dsRNA). The proteins encoded by IFIT1 and IFIT2 are called ISG56 (p56) and ISG54 (p54) in both human and murine species, while the protein encoded by IFIT3 is of a different size in murine (ISG49 or p49) than its human counterpart (ISG60 or p60). Studies conducted in murine species reveals that the three members exhibit distinct properties and induction patterns in different cell types. While all three murine proteins are shown to bind the c-subunit of the translation initiation factor eIF3, p49/Ifit3 does not inhibit protein synthesis as p56/Ifit1 and p54/Ifit2.

Description

Catalogue Number

ABF1048

Description

Anti-IFIT3 Antibody

Alternate Names

Interferon-induced protein with tetratricopeptide repeats 3
IFIT-3
Glucocorticoid-attenuated response gene 49 protein
GARG-49
P49
IRG2
IFIT3

Background Information

Interferon-induced protein with tetratricopeptide repeats 3 (UniProt Q64345; also known as GARG-49, Glucocorticoid-attenuated response gene 49 protein. IFIT-3, IRG2, p49) is encoded by the Ifit3 (also known as Garg49, Ifi49, Isg49) gene (Gene ID 5959) in murine species. IFIT1, IFIT2, and IFIT3 constitute the family of interferon-stimulated genes (ISGs) that are induced in response to virus infection, stimulation by interferons (IFNs), or double-stranded RNA (dsRNA). The proteins encoded by IFIT1 and IFIT2 are called ISG56 (p56) and ISG54 (p54) in both human and murine species, while the protein encoded by IFIT3 is of a different size in murine (ISG49 or p49) than its human counterpart (ISG60 or p60). Studies conducted in murine species reveals that the three members exhibit distinct properties and induction patterns in different cell types. While all three murine proteins are shown to bind the c-subunit of the translation initiation factor eIF3, p49/Ifit3 does not inhibit protein synthesis as p56/Ifit1 and p54/Ifit2.

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.

Product Information

Format

Serum

Presentation

Rabbit polyclonal serum with 0.05% sodium azide.

Applications
Application	This Anti-IFIT3 Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry, Immunocytochemistry for the detection of IFIT3.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry (Paraffin) Flow Cytometry Immunocytochemistry
Application Notes	Western Blotting Analysis: A 1:2,500 dilution of this antibody detected IFIT3 in 40 µg of IFN beta treated RAW264.7 cell lysate (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH). Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in RAW264.7 and wild-type, but not IRF3 or Stat1 knockout, MEFs upon Sendai virus/SeV infection, dsRNA or INF-beta stimulation in cultures, as well as in liver/lung/spleen/colon tissues from mice after dsRNA or INF-alpha i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Western Blotting Analysis: A representative lot detected differential Ifit3/ISG49 inductions between murine MPC podocytes and primary murine glomerular mesangial cells in response to Sendai virus (SeV), encephalomyocarditis virus (EMCV), and vesicular stomatitis virus (VSV) infection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Western Blotting Analysis: A representative lot selectively detected exogenously expressed murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54, in transfected HT1080 cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in IFN-alpha-stimulated primary murine spleen B cells and in IFN-beta-stimulated MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053; Fensterl, V., et al. (2012). PLoS Pathog. 8(5):e1002712). Immunoprecipitation Analysis: A representative lot selectively immunoprecipitated exogenously expressed murine Ifit3/ISG49, but not Ifit1/ISG56, from transfected MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Immunohistochemistry Analysis: A representative lot detected differential Ifit3/ISG49 induction among various cell types in formaldehyde-fixed, paraffin-embedded kidney tissue sections from mice after Sendai virus (SeV) or dsRNA i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Flow Cytometry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 immunoreactivity using murine bone marrow-derived dendritic cells, as well as isolated CD3+ (T cells) and B220+ (B cells) peripheral blood cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Immunocytochemistry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 expression in MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053). Flow Cytometry Analysis: A representative lot (1:500 dilution) detected IFN-beta-induced Ifit3/ISG49 immunoreactivity in primary murine macrophages (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH).

Applications

Application

This Anti-IFIT3 Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry, Immunocytochemistry for the detection of IFIT3.

Key Applications

Western Blotting
Immunoprecipitation
Immunohistochemistry (Paraffin)
Flow Cytometry
Immunocytochemistry

Application Notes

Western Blotting Analysis: A 1:2,500 dilution of this antibody detected IFIT3 in 40 µg of IFN beta treated RAW264.7 cell lysate (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH).
Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in RAW264.7 and wild-type, but not IRF3 or Stat1 knockout, MEFs upon Sendai virus/SeV infection, dsRNA or INF-beta stimulation in cultures, as well as in liver/lung/spleen/colon tissues from mice after dsRNA or INF-alpha i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot detected differential Ifit3/ISG49 inductions between murine MPC podocytes and primary murine glomerular mesangial cells in response to Sendai virus (SeV), encephalomyocarditis virus (EMCV), and vesicular stomatitis virus (VSV) infection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot selectively detected exogenously expressed murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54, in transfected HT1080 cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Western Blotting Analysis: A representative lot detected Ifit3/ISG49 induction in IFN-alpha-stimulated primary murine spleen B cells and in IFN-beta-stimulated MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053; Fensterl, V., et al. (2012). PLoS Pathog. 8(5):e1002712).
Immunoprecipitation Analysis: A representative lot selectively immunoprecipitated exogenously expressed murine Ifit3/ISG49, but not Ifit1/ISG56, from transfected MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Immunohistochemistry Analysis: A representative lot detected differential Ifit3/ISG49 induction among various cell types in formaldehyde-fixed, paraffin-embedded kidney tissue sections from mice after Sendai virus (SeV) or dsRNA i.v. injection (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Flow Cytometry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 immunoreactivity using murine bone marrow-derived dendritic cells, as well as isolated CD3+ (T cells) and B220+ (B cells) peripheral blood cells (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Immunocytochemistry Analysis: A representative lot detected IFN-beta-induced Ifit3/ISG49 expression in MEFs (Fensterl, V., et al. (2008). J Virol. 82(22):11045-11053).
Flow Cytometry Analysis: A representative lot (1:500 dilution) detected IFN-beta-induced Ifit3/ISG49 immunoreactivity in primary murine macrophages (Courtesy of Dr. Ganes C. Sen, Cleveland Clinic, OH).

Biological Information
Immunogen	His-tagged recombinant protein corresponding to mouse IFIT3.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Reacts with murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54.
Species Reactivity	Mouse Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_034631
Gene Symbol	Ifit3 Garg49 Ifi49 Isg49
Purification Method	Unpurified
UniProt Number	Q64345
Molecular Weight	~48 kDa observed

Biological Information

Immunogen

His-tagged recombinant protein corresponding to mouse IFIT3.

Concentration

Please refer to lot specific datasheet.

Host

Rabbit

Specificity

Reacts with murine Ifit3/ISG49 and human IFIT3/ISG60, but not murine Ifit1/ISG56 or Ifit2/ISG54.

Species Reactivity

Mouse
Human

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_034631

Gene Symbol

Ifit3
Garg49
Ifi49
Isg49

Purification Method

Unpurified

UniProt Number

Q64345

Molecular Weight

~48 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in IFN beta treated RAW264.7 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected IFIT3 in 10 µg of IFN beta treated RAW264.7 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting in IFN beta treated RAW264.7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IFIT3 in 10 µg of IFN beta treated RAW264.7 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-IFIT3 (rabbit polyclonal)

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