Anti-B-Raf, clone 4B3.2 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1094
Description	Anti-B-Raf Antibody, clone 4B3.2
Alternate Names	v-raf murine sarcoma viral oncogene homolog B1 murine sarcoma viral (v-raf) oncogene homolog B1 94 kDa B-raf protein B-Raf proto-oncogene serine/threonine-protein kinase (p94)
Background Information	The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a C-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. B-Raf acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. B-Raf is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. B-Raf acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.

Description

Catalogue Number

05-1094

Description

Anti-B-Raf Antibody, clone 4B3.2

Alternate Names

v-raf murine sarcoma viral oncogene homolog B1
murine sarcoma viral (v-raf) oncogene homolog B1
94 kDa B-raf protein
B-Raf proto-oncogene serine/threonine-protein kinase (p94)

Background Information

The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a C-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. B-Raf acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. B-Raf is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. B-Raf acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.

Product Information
Format	Purified
Control	K562 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

K562 cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Detect B-Raf using this Anti-B-Raf Antibody, clone 4B3.2 validated for use in WB & IC.
Key Applications	Western Blotting
Application Notes	Snap I.D. Analysis: 2 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate. Immunocytochemistry Analysis: 1:500 dilution of this antibody has been shown to detect B-Raf in A431 cells.

Applications

Application

Detect B-Raf using this Anti-B-Raf Antibody, clone 4B3.2 validated for use in WB & IC.

Key Applications

Western Blotting

Application Notes

Snap I.D. Analysis: 2 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution of this antibody has been shown to detect B-Raf in A431 cells.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human B-Raf.
Clone	4B3.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes B-Raf.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004324
Entrez Gene Summary	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq].
Gene Symbol	BRAF1 RAFB1 B-Raf B-RAF1 p943
Purification Method	Protein G Purified
UniProt Number	P15056
UniProt Summary	FUNCTION: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. COFACTOR: Binds 2 zinc ions per subunit (By similarity). SIZE: 766 amino acids; 84437 Da SUBUNIT: Interacts with RIT1 (By similarity). SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Brain and testis. DISEASE: Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500]. SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight	84 kDa

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to human B-Raf.

Clone

4B3.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes B-Raf.

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_004324

Entrez Gene Summary

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq].

Gene Symbol

BRAF1
RAFB1
B-Raf
B-RAF1
p943

Purification Method

Protein G Purified

UniProt Number

P15056

UniProt Summary

FUNCTION: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

COFACTOR: Binds 2 zinc ions per subunit (By similarity).

SIZE: 766 amino acids; 84437 Da

SUBUNIT: Interacts with RIT1 (By similarity).

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Brain and testis.

DISEASE: Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].

SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.

Molecular Weight

84 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in K562 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected B-Raf on 10 µg of K562 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-B-Raf, clone 4B3.2 (mouse monoclonal)

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