Anti-IGF-I, clone Sm1.2 Monoclonal Antibody

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, Ch, Av	IHC, IP, NEUT, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, Ch, Av

IHC, IP, NEUT, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-172
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-IGF-I Antibody, clone Sm1.2
Alternate Names	Mechano growth factor insulin-like growth factor 1 insulin-like growth factor 1 (somatomedin C) somatomedin C
Background Information	Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Description

Catalogue Number

05-172

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-IGF-I Antibody, clone Sm1.2

Alternate Names

Mechano growth factor
insulin-like growth factor 1
insulin-like growth factor 1 (somatomedin C)
somatomedin C

Background Information

Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges.
IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Product Information
Format	Purified
Control	MCF-7 cell extracts
Presentation	Purified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA.

Product Information

Format

Purified

Control

MCF-7 cell extracts

Presentation

Purified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA.

Applications
Application	Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB.
Key Applications	Immunohistochemistry Immunoprecipitation Neutralizing Western Blotting
Application Notes	Immunoprecipitation: 5 μg of a previous lot immunoprecipitated 100 ng of IGF-I. Immunohistochemistry: 10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections. (Lui, 1994). Neutralization: 10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).

Applications

Application

Anti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB.

Key Applications

Immunohistochemistry
Immunoprecipitation
Neutralizing
Western Blotting

Application Notes

Immunoprecipitation:
5 μg of a previous lot immunoprecipitated 100 ng of IGF-I.

Immunohistochemistry:
10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections.
(Lui, 1994).

Neutralization:
10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).

Biological Information
Immunogen	Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986).
Clone	Sm1.2
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997).
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat Chicken Avian
Species Reactivity Note	Human, mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF-I due to binding protein interference.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000609
Entrez Gene Summary	The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
Gene Symbol	IGF1 IBP1 IGFI IGF-IA MGF IGF-IB
Purification Method	Protein G Purified
UniProt Number	P05019
UniProt Summary	FUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.\| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. SIZE: 195 amino acids; 21841 Da SUBCELLULAR LOCATION: Secreted. DOMAIN: SwissProt: P05019 DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.\| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.\| P01343 ## Belongs to the insulin family.
Molecular Weight	17 kDa, migrates to ~7 kDa

Biological Information

Immunogen

Human insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986).

Clone

Sm1.2

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

IGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997).

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat
Chicken
Avian

Species Reactivity Note

Human, mouse, rat and possibly chicken.
Occasional difficulty neutralizing rat IGF-I due to binding protein interference.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000609

Entrez Gene Summary

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Gene Symbol

IGF1
IBP1
IGFI
IGF-IA
MGF
IGF-IB

Purification Method

Protein G Purified

UniProt Number

P05019

UniProt Summary

FUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
SIZE: 195 amino acids; 21841 Da
SUBCELLULAR LOCATION: Secreted.
DOMAIN: SwissProt: P05019
DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.| P01343 ## Belongs to the insulin family.

Molecular Weight

17 kDa, migrates to ~7 kDa

Product Usage Statements
Quality Assurance	Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated on IGF-I (Catalog # 01-208) under non-reducing conditions.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-IGF-I, clone Sm1.2 Monoclonal Antibody

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