Anti-Calmodulin

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M, Po, R, Vrt	WB, IP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, H, M, Po, R, Vrt

WB, IP

Purified

Monoclonal Antibody

Description
Catalogue Number	05-173
Replaces	04-1158
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Calmodulin Antibody
Alternate Names	calmodulin 1 calmodulin 1 (phosphorylase kinase, delta) phosphorylase kinase, delta subunit
Background Information	Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

Description

Catalogue Number

05-173

Replaces

04-1158

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Calmodulin Antibody

Alternate Names

calmodulin 1
calmodulin 1 (phosphorylase kinase, delta)
phosphorylase kinase, delta subunit

Background Information

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Use 20 μg per lane for minigels.
Presentation	Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

Product Information

Format

Purified

Control

Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Use 20 μg per lane for minigels.

Presentation

Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

Applications
Application	Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Immunoprecipitation: 4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay: Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.

Applications

Application

Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.

Biological Information
Immunogen	Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.
Epitope	a.a. 128-148
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.
Isotype	IgG1
Species Reactivity	Bovine Human Mouse Pig Rat Vertebrates
Species Reactivity Note	Also reacts to wheat germ. Wide cross-reactivity expected.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_008819
Entrez Gene Summary	Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]
Gene Symbol	CALM1 CALML2 CALML2 CALM3 CALM CaM CALM2 CAM2 CAMC CAM3 DD132 CAMIII PHKD CAMI CAMB CAM CAM1
Purification Method	Protein G Chromatography
UniProt Number	P62158
UniProt Summary	FUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY. SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Ref.20 PTM Ubiquitination results in a strongly decreased activity By similarity. Phosphorylation results in a decreased activity By similarity. MISCELLANEOUS This protein has four functional calcium-binding sites. SEQUENCE SIMILARITIES Belongs to the calmodulin family. Contains 4 EF-hand domains. Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.
Molecular Weight	17 kDa

Biological Information

Immunogen

Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.

Epitope

a.a. 128-148

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.

Isotype

IgG1

Species Reactivity

Bovine
Human
Mouse
Pig
Rat
Vertebrates

Species Reactivity Note

Also reacts to wheat germ. Wide cross-reactivity expected.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_008819

Entrez Gene Summary

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]

Gene Symbol

CALM1
CALML2
CALML2
CALM3
CALM
CaM
CALM2
CAM2
CAMC
CAM3
DD132
CAMIII
PHKD
CAMI
CAMB
CAM
CAM1

Purification Method

Protein G Chromatography

UniProt Number

P62158

UniProt Summary

FUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.

SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Ref.20

PTM Ubiquitination results in a strongly decreased activity By similarity.

Phosphorylation results in a decreased activity By similarity.

MISCELLANEOUS This protein has four functional calcium-binding sites.

SEQUENCE SIMILARITIES Belongs to the calmodulin family.

Contains 4 EF-hand domains.

Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.

Molecular Weight

17 kDa

Product Usage Statements
Quality Assurance	Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts. Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

浏览了该商品的用户最终购买

浏览了该商品的用户还浏览了

特卖

重要规格表

我要咨询