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Anti-ICMT (rabbit polyclonal)

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货号：09-119

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 09-119
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Isoprenylcysteine Carboxymethyl Transferase (ICMT) Antibody
Alternate Names
  • Protein-S-isoprenylcysteine O-methyltransferase
  • Isoprenylcysteine carboxylmethyltransferase
  • Prenylated protein carboxyl methyltransferase
  • PPMT
  • Prenylcysteine carboxyl methyltransferase
  • pcCMT
References
Product Information
Format Affinity Purified
Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 0.15 M NaCl, and 0.1% sodium azide.
Applications
Application Anti-Isoprenylcysteine Carboxymethyl Transferase (ICMT) Antibody detects level of Isoprenylcysteine Carboxymethyl Transferase (ICMT) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to amino acids 266-281 of human isoprenylcysteine carboxymethyl transferase (ICMT).
Epitope Near C-terminus.
Host Rabbit
Specificity This polyclonal antibody targets a sequence near the C-terminus of ICMT.
Isotype IgG
Species Reactivity
  • Human
Species Reactivity Note Human (H). Predicated to react with Canine, Chimpanzee, Mouse, and Rat based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined.
Gene Symbol
  • ICMT
  • PCCMT
  • PCMT
  • PPMT
Purification Method Affinity purified.
UniProt Number
UniProt Summary FUNCTION: SwissProt: O60725 # Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
COFACTOR: Divalent cations. Probably zinc (By similarity).
SIZE: 284 amino acids; 31938 Da
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
SIMILARITY: SwissProt: O60725 ## Belongs to the isoprenylcysteine O-methyltransferase family.
Molecular Weight ~34 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected isoprenylcysteine carboxymethyl transferase (ICMT) in 10 g of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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