Anti-Importin subunit alpha-1, clone 8G5 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1526
Description	Anti-Importin subunit alpha-1 Antibody, clone 8G5
Alternate Names	karyopherin alpha 1 (importin alpha 5) importin alpha 5 importin-alpha-S1 recombination activating gene cohort 2 Karyopherin subunit alpha-1 Nucleoprotein interactor 1 RAG cohort protein 2 karyopherin alpha 1
Background Information	The importin alpha/beta heterodimer facilitates the translocation of hundreds of proteins across the nuclear envelope. Importin alpha binds to proteins containing a classical nuclear localization signal and links them to importin beta ((KPNB1), a protein which ferries the complex through the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Description

Catalogue Number

05-1526

Description

Anti-Importin subunit alpha-1 Antibody, clone 8G5

Alternate Names

karyopherin alpha 1 (importin alpha 5)
importin alpha 5
importin-alpha-S1
recombination activating gene cohort 2
Karyopherin subunit alpha-1
Nucleoprotein interactor 1
RAG cohort protein 2
karyopherin alpha 1

Background Information

The importin alpha/beta heterodimer facilitates the translocation of hundreds of proteins across the nuclear envelope. Importin alpha binds to proteins containing a classical nuclear localization signal and links them to importin beta ((KPNB1), a protein which ferries the complex through the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Product Information
Format	Purified
Control	K562 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

K562 cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-Importin subunit alpha-1 Antibody, clone 8G5 is a mouse monoclonal antibody for detection of Importin subunit alpha-1 also known as karyopherin alpha 1, importin alpha 5, importin-alpha-S1 & has been validated in WB.
Key Applications	Western Blotting

Applications

Application

Anti-Importin subunit alpha-1 Antibody, clone 8G5 is a mouse monoclonal antibody for detection of Importin subunit alpha-1 also known as karyopherin alpha 1, importin alpha 5, importin-alpha-S1 & has been validated in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	Recombinant protein corresponding to human Importin subunit alpha-1.
Epitope	Unknown
Clone	8G5
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes Importin subunit alpha-1.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002255.3
Entrez Gene Summary	Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq].
Gene Symbol	RCH2 NPI-1 SRP1 SRP1-beta IPOA5 KPNA1
Purification Method	Protein G Purified
UniProt Number	P52294
UniProt Summary	FUNCTION: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. SUBUNIT STRUCTURE: Forms a complex with importin subunit beta-1. Interacts with ANP32E by similarity. Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed ubiquitously. DOMAIN: Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import. The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins by similarity. The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding by similarity. SEQUENCE SIMILARITIES: Belongs to the importin alpha family. Contains 10 ARM repeats. Contains 1 IBB domain.
Molecular Weight	~ 60 kDa

Biological Information

Immunogen

Recombinant protein corresponding to human Importin subunit alpha-1.

Epitope

Unknown

Clone

8G5

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes Importin subunit alpha-1.

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_002255.3

Entrez Gene Summary

Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq].

Gene Symbol

RCH2
NPI-1
SRP1
SRP1-beta
IPOA5
KPNA1

Purification Method

Protein G Purified

UniProt Number

P52294

UniProt Summary

FUNCTION: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

SUBUNIT STRUCTURE: Forms a complex with importin subunit beta-1. Interacts with ANP32E by similarity. Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

TISSUE SPECIFICITY: Expressed ubiquitously.

DOMAIN: Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.

The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins by similarity.

The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding by similarity.

SEQUENCE SIMILARITIES: Belongs to the importin alpha family.

Contains 10 ARM repeats.

Contains 1 IBB domain.

Molecular Weight

~ 60 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in K562 cell lysate. Western Blot Analysis: 0.01 µg/ml of this antibody detected Importin subunit alpha-1 on 10 µg of K562 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.01 µg/ml of this antibody detected Importin subunit alpha-1 on 10 µg of K562 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Importin subunit alpha-1, clone 8G5 (mouse monoclonal)

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